PMID- 10517659
OWN - NLM
STAT- MEDLINE
DCOM- 19991109
LR  - 20190910
IS  - 1045-3873 (Print)
IS  - 1045-3873 (Linking)
VI  - 10
IP  - 9
DP  - 1999 Sep
TI  - Sequential change in action potential of rabbit epicardium during and following 
      radiofrequency ablation.
PG  - 1252-61
AB  - INTRODUCTION: Although radiofrequency (RF) catheter ablation is used to treat 
      certain cardiac arrhythmias, little is known regarding transient changes in 
      cellular electrophysiology during and following RF delivery. Optical recordings 
      of action potential (OAP) with voltage-sensitive dyes allow immunity from 
      electrical noise during RF delivery. The purpose of this study was to clarify the 
      possible synergistic effects of both the thermal and electrotonic components of 
      RF ablation. METHODS AND RESULTS: In this study, OAPs were recorded on the 
      epicardium of 16 isolated Langendorff-perfused rabbit hearts within or adjacent 
      to lesions made by RF catheters. Hearts were perfused at room temperature with 
      Tyrode's solution containing 2,3-butanedione monoxime and stained by the 
      voltage-sensitive dye di-4-ANEPPS. OAPs were recorded before, during, and after 
      RF pulses. Within the lesion, the action potential duration at 80% repolarization 
      (APD80) of OAP decreased rapidly during the RF pulse, without recovery following 
      the pulse. In the border zone surrounding the lesion, the RF energy resulted in a 
      rapid decrease in APD80, which recovered promptly after the pulse (recovery time 
      constant: 82 +/- 37 sec). APD80 was nonlinearly related to temperature during the 
      RF ablation and responded faster to RF ablation than to purely thermal injury. 
      CONCLUSION: The application of RF energy results in significant changes in 
      myocardial cellular electrophysiologic properties. The RF energy has a 
      combination of thermal and electrotonic effects on the myocardial tissue. The 
      results of this in vitro study may illustrate the cellular basis for commonly 
      observed phenomena in clinical practice.
FAU - Wu, C C
AU  - Wu CC
AD  - Department of Internal Medicine, National Taiwan University Hospital, Taipei.
FAU - Fasciano, R W 2nd
AU  - Fasciano RW 2nd
FAU - Calkins, H
AU  - Calkins H
FAU - Tung, L
AU  - Tung L
LA  - eng
GR  - 48266/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cardiovasc Electrophysiol
JT  - Journal of cardiovascular electrophysiology
JID - 9010756
SB  - IM
MH  - *Action Potentials
MH  - Animals
MH  - Arrhythmias, Cardiac/surgery
MH  - *Catheter Ablation
MH  - Electrophysiology
MH  - Pericardium/*physiology
MH  - Rabbits
EDAT- 1999/10/12 09:00
MHDA- 2001/03/28 10:01
CRDT- 1999/10/12 09:00
PHST- 1999/10/12 09:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/10/12 09:00 [entrez]
AID - 10.1111/j.1540-8167.1999.tb00303.x [doi]
PST - ppublish
SO  - J Cardiovasc Electrophysiol. 1999 Sep;10(9):1252-61. doi: 
      10.1111/j.1540-8167.1999.tb00303.x.