PMID- 10518460
OWN - NLM
STAT- MEDLINE
DCOM- 19991130
LR  - 20131121
IS  - 0031-6903 (Print)
IS  - 0031-6903 (Linking)
VI  - 119
IP  - 10
DP  - 1999 Oct
TI  - [Analysis of micronuclei induced under hyperthermic conditions in human 
      lymphocyte culture by fluorescence in situ hybridization (FISH) and spectral 
      karyotyping (SKY) methods].
PG  - 763-72
AB  - The spectral karyotyping (SKY) method is a novel molecular cytogenetic technique 
      which simultaneously discerns entire chromosomes. In order to elucidate the 
      origins of micronuclei induced under hyperthermic conditions in human lymphocyte 
      culture, peripheral blood cells were cultured at 40 degrees C or 42 degrees C for 
      3-24 h, using the cytokinesis-block method with cytochalasin B. The induced 
      micronuclei were identified by the fluorescence in situ hybridization (FISH) and 
      SKY methods. At 42 degrees C for more than 6 h, the frequency of occurrence of 
      micronuclei in binucleated cells rose with increasing incubation time. By the 
      FISH method, 83.3% of micronuclei induced in 24 h culture at 42 degrees C were 
      shown to be positive for the human centromeric probes. By the SKY method, each 
      micronucleus induced under the hyperthermic conditions was identified 
      unequivocally and shown to contain a specific chromosome. These results suggest 
      that the micronuclei induced under the hyperthermic conditions in human 
      lymphocyte culture contain chromosomes which do not migrate to the poles at the 
      anaphase of the cell cycle because of the breakdown of the spindle apparatus.
FAU - Komae, N
AU  - Komae N
AD  - Research Laboratory, Toyama Chemical Co., Ltd., Japan.
FAU - Hibino, Y
AU  - Hibino Y
FAU - Sugano, N
AU  - Sugano N
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Yakugaku Zasshi
JT  - Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
JID - 0413613
SB  - IM
MH  - Cells, Cultured
MH  - Chromosome Aberrations
MH  - Chromosomes, Human
MH  - *Cytogenetic Analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/*cytology
MH  - *Micronuclei, Chromosome-Defective
MH  - Spindle Apparatus
MH  - *Temperature
EDAT- 1999/10/13 00:00
MHDA- 1999/10/13 00:01
CRDT- 1999/10/13 00:00
PHST- 1999/10/13 00:00 [pubmed]
PHST- 1999/10/13 00:01 [medline]
PHST- 1999/10/13 00:00 [entrez]
PST - ppublish
SO  - Yakugaku Zasshi. 1999 Oct;119(10):763-72.