PMID- 10521113
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20220409
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 72
IP  - 4
DP  - 1999 Oct
TI  - Screening for abnormalities of chromosomes X, Y, and 18 and for diploidy in 
      spermatozoa from infertile men participating in an in vitro 
      fertilization-intracytoplasmic sperm injection program.
PG  - 696-701
AB  - OBJECTIVE: To evaluate the frequency of disomy (for chromosomes X, Y, and 18) and 
      of diploidy in the spermatozoa of infertile men undergoing intracytoplasmic sperm 
      injection (ICSI). DESIGN: Prospective analysis of sperm nuclei by fluorescence in 
      situ hybridization (FISH). SETTING: University-affiliated IVF-ICSI program. 
      PATIENT(S): Semen samples from 19 patients participating in an IVF-ICSI program. 
      INTERVENTION(S): Semen samples were analyzed and prepared for FISH. MAIN OUTCOME 
      MEASURE(S): Semen parameters were evaluated. The frequency of disomy for 
      chromosomes X, Y, and 18 and the frequency of diploidy were analyzed by FISH. 
      RESULT(S): A total of 9,373 spermatozoa from 19 infertile patients were analyzed 
      and compared with spermatozoa from a control group of 5 healthy men. No 
      differences in the frequency of disomy 18 were found, but statistically 
      significant differences in the incidence of sex chromosome disomy and of diploidy 
      were observed. CONCLUSION(S): The study of sperm nuclei by FISH is useful to 
      improve genetic counseling in infertile patients selected for ICSI.
FAU - Aran, B
AU  - Aran B
AD  - Department of Obstetrics and Gynecology, Institut Universitari Dexeus, Barcelona, 
      Spain.
FAU - Blanco, J
AU  - Blanco J
FAU - Vidal, F
AU  - Vidal F
FAU - Vendrell, J M
AU  - Vendrell JM
FAU - Egozcue, S
AU  - Egozcue S
FAU - Barri, P N
AU  - Barri PN
FAU - Egozcue, J
AU  - Egozcue J
FAU - Veiga, A
AU  - Veiga A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations/*diagnosis
MH  - *Chromosome Disorders
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Diploidy
MH  - Fertilization in Vitro
MH  - *Genetic Testing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Male/*genetics
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Reference Values
MH  - Sperm Injections, Intracytoplasmic
MH  - X Chromosome/*genetics
MH  - Y Chromosome/*genetics
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - S0015-0282(99)00307-6 [pii]
AID - 10.1016/s0015-0282(99)00307-6 [doi]
PST - ppublish
SO  - Fertil Steril. 1999 Oct;72(4):696-701. doi: 10.1016/s0015-0282(99)00307-6.