PMID- 10521695
OWN - NLM
STAT- MEDLINE
DCOM- 19991027
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 445
IP  - 1
DP  - 1999 Sep 15
TI  - Anticlastogenic effects of Ginkgo biloba extract (EGb 761) and some of its 
      constituents in irradiated rats.
PG  - 99-104
AB  - In a previous study we reported that radiation-induced clastogenic factors (CF) 
      are found in the plasma of Chernobyl accident recovery workers and that their 
      chromosome damaging effects are inhibited by antioxidant treatment with a Ginkgo 
      biloba extract (EGb761). In the present study, we induced CF in rats with a 
      radiation dose of 4.5 Gy. The protective effects of the complete extract were 
      compared to those obtained with the extract devoid of its terpene fraction 
      (CP205), with isolated ginkgolides A+B and bilobalide at the concentrations 
      present in EGb761. The pretreatment samples were taken at day 22 postirradiation, 
      the posttreatment samples the day following arrest of the 3-week treatment. The 
      adjusted clastogenic score (ACS) were reduced from 11.71+/-3.55 to 2.00+/-2.83 
      after treatment with 100 mg/kg and from 13.43+/-2.23 to 4.29+/-2.14 with 50 mg/kg 
      of the complete extract (p<0.0001). Similar protective effects were observed with 
      CP205, ginkgolides and bilobalide (p<0. 001), while the reduction of ACS in 
      placebo-treated rats was not statistically significant (12.80+/-1.79 and 
      9.20+/-2.68). However, if the efficacy of the treatment was compared to placebo, 
      only the complete extract was significantly protective. While all components 
      exerted anticlastogenic effects at the concentrations present in the complete 
      extract, the comparison of the different groups by analysis of variance did not 
      reveal significant differences. This may be due to to the small number of animals 
      available in each treatment group. The complete extract reduced the ACS by 83% at 
      the dose of 100 mg/kg, while the lower dose of 50 mg/kg and the three components 
      reached only 66%-68% reduction. The better protection provided by the complete 
      extract is due to synergistic rather than to additive effects.
FAU - Alaoui-Youssefi, A
AU  - Alaoui-Youssefi A
AD  - Institut Sante et Developpement, Universite Paris VI, 15-21 Rue de l'Ecole de 
      Medecine, 75006, Paris, France.
FAU - Lamproglou, I
AU  - Lamproglou I
FAU - Drieu, K
AU  - Drieu K
FAU - Emerit, I
AU  - Emerit I
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Diterpenes)
RN  - 0 (Flavonoids)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Ginkgolides)
RN  - 0 (Lactones)
RN  - 0 (Plant Extracts)
RN  - 19FUJ2C58T (Ginkgo biloba extract)
RN  - DF149B9460 (ginkgolide B)
SB  - IM
MH  - Animals
MH  - Antimutagenic Agents/*pharmacology
MH  - Chromosome Aberrations
MH  - *Diterpenes
MH  - Flavonoids/*pharmacology
MH  - Free Radical Scavengers/pharmacology
MH  - Ginkgo biloba/chemistry
MH  - Ginkgolides
MH  - Humans
MH  - Lactones/pharmacology
MH  - Male
MH  - Mitotic Index
MH  - Mutagenicity Tests
MH  - Plant Extracts/pharmacology
MH  - Plants, Medicinal
MH  - Plasma/*drug effects/*radiation effects
MH  - Rats
MH  - Rats, Wistar
MH  - Whole-Body Irradiation
EDAT- 1999/10/16 00:00
MHDA- 1999/10/16 00:01
CRDT- 1999/10/16 00:00
PHST- 1999/10/16 00:00 [pubmed]
PHST- 1999/10/16 00:01 [medline]
PHST- 1999/10/16 00:00 [entrez]
AID - S1383571899001394 [pii]
AID - 10.1016/s1383-5718(99)00139-4 [doi]
PST - ppublish
SO  - Mutat Res. 1999 Sep 15;445(1):99-104. doi: 10.1016/s1383-5718(99)00139-4.