PMID- 10523572
OWN - NLM
STAT- MEDLINE
DCOM- 19991130
LR  - 20200724
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 37
IP  - 11
DP  - 1999 Nov
TI  - Detection of human herpesvirus 6 by reverse transcription-PCR.
PG  - 3672-5
AB  - The role of human herpesvirus 6 (HHV-6) in disease beyond primary infection 
      remains unclear. We have developed and validated a new reverse transcription-PCR 
      (RT-PCR) assay for HHV-6 that can determine the presence of HHV-6 in clinical 
      specimens and differentiate between latent and replicating virus. Peripheral 
      blood mononuclear cells from 109 children were evaluated for HHV-6 by RT-PCR, DNA 
      PCR, and viral culture. Of these samples, 106 were suitable for analysis. A total 
      of 20 samples were positive for HHV-6 by culture and DNA PCR, of which 19 were 
      positive by RT-PCR (sensitivity, 95%). All 28 samples from children that were 
      negative by viral culture, but positive by DNA PCR, were negative for viral 
      transcripts by our RT-PCR assay. One positive RT-PCR result was observed in 56 
      samples that were negative by tissue culture and DNA PCR. This indicates a low 
      rate of false-positive results (1.2%) and a specificity of 98.8%. This RT-PCR 
      assay can reliably differentiate between latent and actively replicating HHV-6 
      and should allow insight into the pathogenesis of this ubiquitous virus.
FAU - Norton, R A
AU  - Norton RA
AD  - Department of Microbiology and Immunology, University of Rochester Medical 
      Center, Rochester, New York 14642, USA. Renee_Norton@urmc.rochester.edu
FAU - Caserta, M T
AU  - Caserta MT
FAU - Hall, C B
AU  - Hall CB
FAU - Schnabel, K
AU  - Schnabel K
FAU - Hocknell, P
AU  - Hocknell P
FAU - Dewhurst, S
AU  - Dewhurst S
LA  - eng
GR  - M01 RR000044/RR/NCRR NIH HHS/United States
GR  - 5 MO1 RR00044/RR/NCRR NIH HHS/United States
GR  - KO4 AI01240/AI/NIAID NIH HHS/United States
GR  - R01 AI33020-06/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - Child, Preschool
MH  - DNA Primers/genetics
MH  - DNA, Viral/analysis/genetics
MH  - Diagnostic Errors
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Herpesviridae Infections/diagnosis/*virology
MH  - Herpesvirus 6, Human/*genetics/*isolation & purification/physiology
MH  - Humans
MH  - Infant
MH  - Male
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods/statistics & numerical 
      data
MH  - Sensitivity and Specificity
MH  - Virology/methods
MH  - Virus Cultivation/methods
MH  - Virus Replication
PMC - PMC85721
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
PMCR- 1999/11/01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
PHST- 1999/11/01 00:00 [pmc-release]
AID - 0690 [pii]
AID - 10.1128/JCM.37.11.3672-3675.1999 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1999 Nov;37(11):3672-5. doi: 10.1128/JCM.37.11.3672-3675.1999.