PMID- 10524203
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20200313
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1446
IP  - 3
DP  - 1999 Sep 3
TI  - Primary structure, gene expression and chromosomal mapping of rodent homologs of 
      the MEN1 tumor suppressor gene.
PG  - 286-94
AB  - Mutations of the MEN1 tumor suppressor gene cause the multiple endocrine 
      neoplasia type 1 (MEN1) syndrome in humans, and they are involved in a variety of 
      sporadic human endocrine tumors. We here characterize the MEN1 gene homologs of 
      the mouse and rat. cDNA was isolated from a mouse phage library, and two 
      alternative MEN1 mRNA transcripts containing variant 5' untranslated regions were 
      identified by RT-PCR in several mouse and rat tissues. When compared to the human 
      molecule, mouse and rat MEN1 (611 and 610 amino acids, respectively) show an 
      overall identity of 96.5% and 97.0% at the protein level, delimiting four 
      conservational domains (A-D). Mouse and rat MEN1 mRNA, as studied by 
      template-calibrated quantitative RT-PCR, is non-exclusively expressed in 
      hematopoietic and endocrine cells, with similar expression patterns found in 
      fetal and adult tissues. Fluorescent in situ hybridization maps the single murine 
      MEN1 locus to chromosome 19, region B. No MEN1 gene mutations were identified in 
      endocrine islet tumor cell lines RIN 5AH (rat) and NIT-1 (mouse) as compared to 
      wild type cDNA. Our data define mouse and rat MEN1 as widely expressed and highly 
      conserved homologs of the human MEN1 tumor suppressor gene whose role in biology 
      and endocrine tumorigenesis is due for experimental study.
FAU - Karges, W
AU  - Karges W
AD  - Department of Internal Medicine, University of Ulm, Germany.
FAU - Maier, S
AU  - Maier S
FAU - Wissmann, A
AU  - Wissmann A
FAU - Dralle, H
AU  - Dralle H
FAU - Dosch, H M
AU  - Dosch HM
FAU - Boehm, B O
AU  - Boehm BO
LA  - eng
SI  - GENBANK/AF130368
SI  - GENBANK/AF130369
SI  - GENBANK/AF130370
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Complementary)
RN  - 0 (Men1 protein, rat)
RN  - 0 (Peptide Library)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - DNA Probes
MH  - DNA, Complementary/chemistry
MH  - Gene Expression Regulation
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - Peptide Library
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Nucleic Acid
MH  - *Transcription Factors
MH  - Tumor Cells, Cultured
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - S0167-4781(99)00089-5 [pii]
AID - 10.1016/s0167-4781(99)00089-5 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Sep 3;1446(3):286-94. doi: 
      10.1016/s0167-4781(99)00089-5.