PMID- 10524204
OWN - NLM
STAT- MEDLINE
DCOM- 19991104
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1446
IP  - 3
DP  - 1999 Sep 3
TI  - Biochemical analysis of mouse FKBP60, a novel member of the FKPB family.
PG  - 295-307
AB  - We have identified mouse and human FKBP60, a new member of the FKBP gene family. 
      FKBP60 shares strongest homology with FKBP65 and SMAP. FKBP60 contains a 
      hydrophobic signal peptide at the N-terminus, 4 peptidyl-prolyl cis/trans 
      isomerase (PPIase) domains and an endoplasmic reticulum retention motif (HDEL) at 
      the C-terminus. Immunodetection of HA-tagged FKBP60 in NIH-3T3 cells suggests 
      that FKBP60 is segregated to the endoplasmic reticulum. Northern blot analysis 
      shows that FKBP60 is predominantly expressed in heart, skeletal muscle, lung, 
      liver and kidney. With N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide as a substrate, 
      recombinant GST-FKBP60 is shown to accelerate effectively the isomerization of 
      the peptidyl-prolyl bond. This isomerization activity is inhibited by FK506. 
      mFKBP60 binds Ca2+ in vitro, presumably by its C-terminal EF-hand Ca2+ binding 
      motif, and is phosphorylated in vivo. hFKBP60 has been mapped to 7p12 and/or 7p14 
      by fluorescence in situ hybridization (FISH).
FAU - Shadidy, M
AU  - Shadidy M
AD  - Department of Molecular Genetics, University of Tromso, Norway.
FAU - Caubit, X
AU  - Caubit X
FAU - Olsen, R
AU  - Olsen R
FAU - Seternes, O M
AU  - Seternes OM
FAU - Moens, U
AU  - Moens U
FAU - Krauss, S
AU  - Krauss S
LA  - eng
SI  - GENBANK/AF089745
SI  - GENBANK/AF090334
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - 0 (FKBP9 protein, human)
RN  - 0 (Fkbp9 protein, mouse)
RN  - 0 (Protein Sorting Signals)
RN  - EC 5.2.1.- (Tacrolimus Binding Proteins)
RN  - EC 5.2.1.8 (Immunophilins)
RN  - EC 5.2.1.8 (Peptidylprolyl Isomerase)
RN  - SY7Q814VUP (Calcium)
RN  - WM0HAQ4WNM (Tacrolimus)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Calcium/metabolism
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA, Complementary/isolation & purification
MH  - Endoplasmic Reticulum/metabolism/ultrastructure
MH  - Gene Expression
MH  - Humans
MH  - Immunophilins/chemistry/*genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptidylprolyl Isomerase/antagonists & inhibitors/chemistry
MH  - Phosphorylation
MH  - Protein Sorting Signals/chemistry
MH  - Sequence Homology, Amino Acid
MH  - Tacrolimus/pharmacology
MH  - *Tacrolimus Binding Proteins
EDAT- 1999/10/19 00:00
MHDA- 1999/10/19 00:01
CRDT- 1999/10/19 00:00
PHST- 1999/10/19 00:00 [pubmed]
PHST- 1999/10/19 00:01 [medline]
PHST- 1999/10/19 00:00 [entrez]
AID - S0167-4781(99)00080-9 [pii]
AID - 10.1016/s0167-4781(99)00080-9 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Sep 3;1446(3):295-307. doi: 
      10.1016/s0167-4781(99)00080-9.