PMID- 10525437
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20171116
IS  - 1046-2023 (Print)
IS  - 1046-2023 (Linking)
VI  - 19
IP  - 1
DP  - 1999 Sep
TI  - Local lymph node assay: differentiating allergic and irritant responses using 
      flow cytometry.
PG  - 48-55
AB  - The murine local lymph node assay (LLNA) is a method for assessing the contact 
      sensitization potential of chemicals. Based on events that occur during the 
      induction phase of a contact sensitization response, the LLNA measures the in 
      vivo proliferation of cells in the draining lymph nodes (DLNs) of mice following 
      topical exposure to chemicals. In terms of predictive identification of important 
      skin sensitizers, the LLNA has been shown to be at least as sensitive as, and 
      much more reliable than, current guinea pig tests. However, proliferation has 
      also been observed following treatment with some irritants. In an attempt to 
      distinguish allergic from irritant-induced proliferation, flow cytometric 
      techniques have been used to examine the phenotype of lymphocyte subsets in the 
      DLNs as well as markers of T-lymphocyte activation and memory. Mice were treated 
      on the ears for 3 consecutive days with allergens or irritants. The DLNs were 
      harvested 72 h after the final treatment. Single-cell suspensions were prepared, 
      counted, and stained for analysis of the percentages of T cells and B cells and 
      T-cell expression of two adhesion molecules that have been associated with 
      differentiating naive and activated/memory T cells, CD62L (L-selectin) and CD44 
      (H-cam). Increases in lymph node cellularity were observed in both allergen- and 
      irritant-treated mice relative to naive and vehicle-treated animals. Mice treated 
      with allergens showed a preferential increase in the percentage of B220(+) B 
      cells compared with irritant-treated mice. Treatment with allergens, but not 
      irritants, resulted in a selective increase in the percentages of CD4(+) and 
      CD8(+) cells expressing the T-cell activation/memory phenotype CD62L(lo)CD44(hi). 
      Taken together, flow cytometric analysis of cell phenotype and expression of 
      T-cell activation/memory markers may provide important information for 
      differentiating allergen- and irritant-induced proliferative responses in the 
      DLNs of chemically treated mice.
CI  - Copyright 1999 Academic Press.
FAU - Gerberick, G F
AU  - Gerberick GF
AD  - Miami Valley Laboratories, The Procter and Gamble Company, Cincinnati, Ohio 
      45239-8707, USA. gerberick.gf@pg.com
FAU - Cruse, L W
AU  - Cruse LW
FAU - Ryan, C A
AU  - Ryan CA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
RN  - 0 (Allergens)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (Irritants)
RN  - 126880-86-2 (L-Selectin)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animals
MH  - B-Lymphocytes/drug effects/immunology
MH  - CD4-Positive T-Lymphocytes/drug effects/immunology
MH  - CD8-Positive T-Lymphocytes/drug effects/immunology
MH  - Dermatitis, Contact/etiology/immunology
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Guinea Pigs
MH  - Hyaluronan Receptors/biosynthesis
MH  - Irritants/*toxicity
MH  - L-Selectin/biosynthesis
MH  - Lymph Nodes/cytology/*drug effects/immunology
MH  - Lymphocyte Activation/drug effects
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Phenotype
EDAT- 1999/10/20 00:00
MHDA- 1999/10/20 00:01
CRDT- 1999/10/20 00:00
PHST- 1999/10/20 00:00 [pubmed]
PHST- 1999/10/20 00:01 [medline]
PHST- 1999/10/20 00:00 [entrez]
AID - S1046-2023(99)90826-5 [pii]
AID - 10.1006/meth.1999.0826 [doi]
PST - ppublish
SO  - Methods. 1999 Sep;19(1):48-55. doi: 10.1006/meth.1999.0826.