PMID- 10525450
OWN - NLM
STAT- MEDLINE
DCOM- 19991122
LR  - 20071115
IS  - 1046-2023 (Print)
IS  - 1046-2023 (Linking)
VI  - 19
IP  - 1
DP  - 1999 Sep
TI  - Dendritic cells as natural adjuvants.
PG  - 142-7
AB  - Dendritic cells (DCs) are professional antigen presenting cells that hold the key 
      to the induction of T-cell responses. Therefore, the use of DCs for immunotherapy 
      to stimulate immune responses has recently raised a great deal of interest. Many 
      clinical trials using DCs have been initiated to stimulate immune responses 
      against tumors or infectious agents. Several issues need to be considered before 
      DCs can be used successfully as natural adjuvants: DCs have to be generated in 
      sufficient numbers; they should display morphological, phenotypical, and 
      functional properties of DCs; and they should be able to present antigens. In the 
      present review we focus on methods for the purification of DCs from human bone 
      marrow and peripheral blood and for the optimization of in vitro cell culture 
      systems. Methods to generate growth factor-dependent mouse DC lines are also 
      described.
CI  - Copyright 1999 Academic Press.
FAU - Citterio, S
AU  - Citterio S
AD  - Department of Biotechnology and Biological Sciences, University of 
      Milano-Bicocca, Milan, I-20129, Italy.
FAU - Rescigno, M
AU  - Rescigno M
FAU - Foti, M
AU  - Foti M
FAU - Granucci, F
AU  - Granucci F
FAU - Aggujaro, D
AU  - Aggujaro D
FAU - Gasperi, C
AU  - Gasperi C
FAU - Matyszak, M K
AU  - Matyszak MK
FAU - Girolomoni, G
AU  - Girolomoni G
FAU - Ricciardi-Castagnoli, P
AU  - Ricciardi-Castagnoli P
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Growth Substances)
SB  - IM
MH  - *Adjuvants, Immunologic
MH  - Animals
MH  - Blood Cells/cytology/immunology
MH  - Bone Marrow Cells/cytology/immunology
MH  - Cell Line
MH  - Cell Separation/methods
MH  - Clinical Trials as Topic
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Growth Substances/administration & dosage
MH  - Humans
MH  - Immunotherapy/methods
MH  - Mice
MH  - Neoplasms/immunology/therapy
RF  - 41
EDAT- 1999/10/20 00:00
MHDA- 1999/10/20 00:01
CRDT- 1999/10/20 00:00
PHST- 1999/10/20 00:00 [pubmed]
PHST- 1999/10/20 00:01 [medline]
PHST- 1999/10/20 00:00 [entrez]
AID - S1046-2023(99)90839-3 [pii]
AID - 10.1006/meth.1999.0839 [doi]
PST - ppublish
SO  - Methods. 1999 Sep;19(1):142-7. doi: 10.1006/meth.1999.0839.