PMID- 10526139
OWN - NLM
STAT- MEDLINE
DCOM- 19991221
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 842
IP  - 2
DP  - 1999 Sep 25
TI  - Neurotrophin-elicited short-term glutamate release from cultured cerebellar 
      granule neurons.
PG  - 431-8
AB  - Brain-derived neurotrophic factor (BDNF) has been suggested to play an important 
      role in neuronal plasticity. In this study, we investigated the effects of BDNF 
      on short-term transmitter release from cultured CNS neurons. Rapid and transient 
      glutamate and aspartate releases induced by BDNF were observed from cultured 
      cortical, hippocampal, striatal and cerebellar neurons. We furthermore 
      investigated the mechanism of release induced by neurotrophins from cerebellar 
      granule cells, since granule cells represent a large homogeneous glutamatergic 
      population. NGF and NT-3 elicited neurotrophin-induced release of glutamate as 
      well as BDNF from the cerebellar granule neurons. The release was dependent on 
      intracellular Ca(2+) mobilization. Pretreatment with K252a and also TrkB-IgG 
      completely blocked the glutamate and aspartate release elicited by BDNF, but not 
      by NGF. The cerebellar granule neurons expressed trkB and p75 mRNAs at high 
      levels, but not trkA mRNA. These results suggested that while BDNF induced 
      release via TrkB, NGF-elicited release was not mediated by Trks. Furthermore, in 
      the experiment using the styryl dye FM1-43, which selectively labels synaptic 
      vesicles, neither BDNF nor NGF evoked dye loss, suggesting that 
      neurotrophin-induced excitatory amino acid release occurs through a 
      non-exocytotic pathway.
FAU - Numakawa, T
AU  - Numakawa T
AD  - Division of Protein Biosynthesis, Institute for Protein Research, Osaka 
      University, Suita, Osaka, Japan.
FAU - Takei, N
AU  - Takei N
FAU - Yamagishi, S
AU  - Yamagishi S
FAU - Sakai, N
AU  - Sakai N
FAU - Hatanaka, H
AU  - Hatanaka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 139890-68-9 (1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid 
      acetoxymethyl ester)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 67526-95-8 (Thapsigargin)
RN  - A510CA4CBT (Proadifen)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology/physiology
MH  - Calcium/metabolism
MH  - Cells, Cultured
MH  - Cerebellum/cytology/*physiology
MH  - Cerebral Cortex/cytology/physiology
MH  - Egtazic Acid/analogs & derivatives/pharmacology
MH  - Embryo, Mammalian
MH  - Exocytosis
MH  - Hippocampus/cytology/physiology
MH  - Nerve Growth Factors/*pharmacology
MH  - Neurons/cytology/drug effects/*physiology
MH  - Neurotrophin 3/pharmacology
MH  - Proadifen/pharmacology
MH  - Rats
MH  - Receptor, trkB/antagonists & inhibitors/physiology
MH  - Thapsigargin/pharmacology
EDAT- 1999/10/20 00:00
MHDA- 1999/10/20 00:01
CRDT- 1999/10/20 00:00
PHST- 1999/10/20 00:00 [pubmed]
PHST- 1999/10/20 00:01 [medline]
PHST- 1999/10/20 00:00 [entrez]
AID - S0006-8993(99)01867-3 [pii]
AID - 10.1016/s0006-8993(99)01867-3 [doi]
PST - ppublish
SO  - Brain Res. 1999 Sep 25;842(2):431-8. doi: 10.1016/s0006-8993(99)01867-3.