PMID- 10526316
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20191103
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 41
IP  - 3
DP  - 1999 Nov 15
TI  - Parvalbumin immunoreactivity is enhanced by brain-derived neurotrophic factor in 
      organotypic cultures of rat retina.
PG  - 376-84
AB  - The rodent retina undergoes considerable postnatal neurogenesis and phenotypic 
      differentiation, and it is likely that diffusible neurotrophic factors contribute 
      to this development and to the subsequent formation of functional retinal 
      circuitry. Accordingly, perturbation of specific neurotrophin ligand-receptor 
      interactions has provided valuable information as to the fundamental processes 
      underlying this development. In the present studies we have built upon our 
      previous observation that suppression of expression of trk(B), the high-affinity 
      receptor for brain-derived neurotrophic factor (BDNF), in the postnatal rat 
      retina results in the alteration of a specific interneuron in the rod pathway-the 
      parvalbumin (PV)-immunoreactive AII amacrine cell. Here, we isolated retinas from 
      newborn rats and maintained them in organotypic culture for up to 14 days 
      (approximating the time of eye opening, in vivo) in the presence of individual 
      neurotrophins [BDNF or nerve growth factor (NGF)]. We then examined histological 
      sections of cultures for PV immunoreactivity. In control cultures, only sparse 
      PV-immunostained cells were observed. In cultures supplemented with NGF, numerous 
      lightly immunostained somata were present in the inner nuclear layer (INL) at the 
      border of the inner plexiform layer (IPL). Many of these cells had rudimentary 
      dendritic arborizations in the IPL. Cultures supplemented with BDNF displayed 
      numerous well-immunostained somata at the INL/IPL border that gave rise to 
      elaborate dendritic arborizations that approximated the morphology of mature AII 
      amacrine cells in vivo. These observations indicate that neurotrophins have 
      specific effects upon the neurochemical and, perhaps, morphological 
      differentiation of an important interneuron in a specific functional retinal 
      circuit.
CI  - Copyright 1999 John Wiley & Sons, Inc.
FAU - Rickman, D W
AU  - Rickman DW
AD  - Department of Ophthalmology and Visual Sciences, University of Iowa College of 
      Medicine, 200 Hawkins Drive, Iowa City, Iowa 52242-1091, USA.
LA  - eng
GR  - EY 11389/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Parvalbumins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology/physiology
MH  - Immunohistochemistry
MH  - Organ Culture Techniques
MH  - Parvalbumins/*analysis/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/*chemistry/cytology/*growth & development
MH  - Retinal Ganglion Cells/*chemistry/drug effects/physiology
EDAT- 1999/10/20 00:00
MHDA- 1999/10/20 00:01
CRDT- 1999/10/20 00:00
PHST- 1999/10/20 00:00 [pubmed]
PHST- 1999/10/20 00:01 [medline]
PHST- 1999/10/20 00:00 [entrez]
AID - 10.1002/(SICI)1097-4695(19991115)41:3<376::AID-NEU6>3.0.CO;2-F [pii]
AID - 10.1002/(sici)1097-4695(19991115)41:3<376::aid-neu6>3.0.co;2-f [doi]
PST - ppublish
SO  - J Neurobiol. 1999 Nov 15;41(3):376-84. doi: 
      10.1002/(sici)1097-4695(19991115)41:3<376::aid-neu6>3.0.co;2-f.