PMID- 10526537
OWN - NLM
STAT- MEDLINE
DCOM- 19991102
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 114
IP  - 1
DP  - 1999 Oct 1
TI  - Chromosomal aberrations in Bilharzial bladder cancer as detected by fluorescence 
      in situ hybridization.
PG  - 62-7
AB  - Cancer of the bladder is a frequent malignancy in Egypt and other developing 
      countries in which bladder infection with the parasite Schistosoma haematobium is 
      common. Several epidemiological, histopathological, and clinical characteristics 
      of cancer of the Bilharzial bladder suggest that it is distinct from bladder 
      cancer seen in other places in the world. No numerical aberrations of chromosomes 
      that might be specific for Bilharzial bladder carcinoma have been established. In 
      this study, we used fluorescence in situ hybridization (FISH) with 
      centromere-specific probes for chromosomes 3, 4, 7, 8, 9, 10, 11, 16, and 17 to 
      detect numerical aberrations of these chromosomes in frozen-stored samples of 31 
      Egyptian patients affected with Bilharzial carcinoma. Among 5 types of 
      chromosomes examined, imbalance was observed; the most common imbalance was a 
      loss of chromosome 9 (48.4%), with numerical aberration of chromosome 17 being 
      the second most-frequent anomaly (19.4%). The presence of such anomalies, 
      especially losses of chromosome 9, are associated with a younger age group of 
      patients, as well as with a lower grade tumor and negative pelvic node 
      involvement by the disease. Fluorescence in situ hybridization analysis thus 
      proved to be a useful method for detecting numerical aberrations of individual 
      chromosomes, with application to touch preparations of frozen-stored tissue 
      having the advantage of exact sampling of cancer foci. This result also suggests 
      that the mechanism of genetic progression of bladder cancer is independent of its 
      etiology.
FAU - Aly, M S
AU  - Aly MS
AD  - Faculty of Science, National Cancer Institute, Cairo University, Egypt.
FAU - Khaled, H M
AU  - Khaled HM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 9
MH  - Egypt
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 1999/10/20 00:00
MHDA- 1999/10/20 00:01
CRDT- 1999/10/20 00:00
PHST- 1999/10/20 00:00 [pubmed]
PHST- 1999/10/20 00:01 [medline]
PHST- 1999/10/20 00:00 [entrez]
AID - S0165460899000400 [pii]
AID - 10.1016/s0165-4608(99)00040-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Oct 1;114(1):62-7. doi: 
      10.1016/s0165-4608(99)00040-0.