PMID- 10528245
OWN - NLM
STAT- MEDLINE
DCOM- 19991201
LR  - 20220129
IS  - 0148-7299 (Print)
IS  - 0148-7299 (Linking)
VI  - 87
IP  - 1
DP  - 1999 Nov 5
TI  - Molecular cytogenetic resources specific for chromosome 12.
PG  - 40-4
AB  - We have generated a panel of 20 somatic cell hybrids retaining fragments of human 
      chromosome 12. Each hybrid was characterized cytogenetically by reverse 
      fluorescence in situ hybridization (FISH) and molecularly by 24 sequence tagged 
      sites (STSs) spaced evenly along the chromosome. The panel can be exploited to 
      map subregionally DNA sequences on chromosome 12 and to generate partial 
      chromosome paints useful in the characterization of chromosomal rearrangements 
      involving this chromosome. Furthermore, a panel of 58 yeast artificial 
      chromosomes (YACs) mapping to chromosome 12 was characterized by FISH experiments 
      on normal human metaphases. A subset of this panel is recognized by the STSs used 
      in the somatic cell hybrid characterization. In this way a correlation between 
      the genetic and the physical maps of this chromosome can be established.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Viggiano, L
AU  - Viggiano L
AD  - Institute of Genetics, University of Bari, Bari, Italy.
FAU - Miolla, V
AU  - Miolla V
FAU - Ricco, A
AU  - Ricco A
FAU - Storlazzi, C T
AU  - Storlazzi CT
FAU - Pennacchia, M
AU  - Pennacchia M
FAU - Fratello, A
AU  - Fratello A
FAU - Mancini, M
AU  - Mancini M
FAU - Marzella, R
AU  - Marzella R
FAU - Archidiacono, N
AU  - Archidiacono N
FAU - Rocchi, M
AU  - Rocchi M
LA  - eng
GR  - E.0672/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Med Genet
JT  - American journal of medical genetics
JID - 7708900
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - Cricetinae
MH  - *Cytogenetic Analysis
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Sequence Tagged Sites
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
AID - 10.1002/(SICI)1096-8628(19991105)87:1<40::AID-AJMG8>3.0.CO;2-2 [pii]
PST - ppublish
SO  - Am J Med Genet. 1999 Nov 5;87(1):40-4.