PMID- 10528804
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20190826
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 69
IP  - 3
DP  - 1999 Sep 1
TI  - The human immunoglobulin variable lambda locus IGLV9 gene is a monomorphic marker 
      in the urban Brazilian population.
PG  - 369-70
AB  - The physical map of the human immunoglobulin variable lambda locus (IGLV) located 
      on chromosome 22q11.1-q11.2 shows the existence of 52 functional V-lambda genes 
      distributed among three V-clusters. The IGLV9S1 gene, located in the V-B cluster, 
      is a sequence tagged site and is a useful marker for restriction fragment length 
      polymorphism (RFLP) population studies. The V-lambda genes are associated in the 
      genome with EcoRI fragments detectable in Southern blots of genomic DNA samples. 
      We have analysed DNA samples of an urban Brazilian population by 
      Southern-EcoRI-RFLP using an IGLV9 gene segment. Among 75 unrelated individuals 
      analysed, we detected a single 6.0 kb EcoRI fragment containing the IGLV9 gene at 
      100% frequency. Reverse transcription followed by polymerase chain reaction 
      (RT-PCR) of peripheral blood leukocyte total RNA from unrelated individuals 
      showed that IGLV9S1 is a functional gene contributing to the B lymphocyte 
      repertoire. These data represent evidence for monomorphism of the IGVL9S1 gene in 
      this urban population. We demonstrate that IGLV9S1 is a functional single copy 
      gene and is an important marker in the IGLV locus.
FAU - da Silva, M I
AU  - da Silva MI
AD  - Departamento de Genetica, Faculdade de Medicina de Ribeirao Preto, Universidade 
      de Sao Paulo, Brazil.
FAU - Passos, G A
AU  - Passos GA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Biomarkers)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Immunoglobulin lambda-Chains)
SB  - IM
MH  - Biomarkers
MH  - Brazil
MH  - Chromosomes, Human, Pair 22
MH  - Genes, Immunoglobulin
MH  - Humans
MH  - Immunoglobulin Variable Region/*genetics
MH  - Immunoglobulin lambda-Chains/*genetics
MH  - Polymorphism, Restriction Fragment Length
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
AID - S0165247899001091 [pii]
AID - 10.1016/s0165-2478(99)00109-1 [doi]
PST - ppublish
SO  - Immunol Lett. 1999 Sep 1;69(3):369-70. doi: 10.1016/s0165-2478(99)00109-1.