PMID- 10528920
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20190910
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 75
IP  - 9
DP  - 1999 Sep
TI  - Induction and persistence of chromosomal exchanges in mouse bone marrow cells 
      following whole-body exposure to X-rays.
PG  - 1119-28
AB  - PURPOSE: To investigate the induction and persistence of chromosome aberrations 
      in mouse bone marrow cells after X-ray exposure and to detect differential 
      involvement of individual chromosomes in translocations. MATERIALS AND METHODS: 
      Male and female Swiss mice were exposed to 1 and 3 Gy of X-rays. Chromosome 
      aberrations in bone marrow cells were analysed at 1, 7, 21 and 100 days following 
      irradiation by means of fluorescence in situ hybridization (FISH) with mouse 
      chromosome-specific DNA libraries (#1,13; #2,8; #6,15 and X,Y). In total, 38% of 
      mouse genome was painted and examined. RESULTS: Pooled data indicate that the 
      frequencies of dicentrics and fragments decreased with time and reached to the 
      control level at day 21 after exposure. Following exposure to 1 Gy of X-rays, the 
      frequencies of translocations were not significantly lower between days 7 and 100 
      than observed at day 1. However, the frequencies of translocations for the 3 Gy 
      group were significantly (about 40%) lower at day 7, then remained constant up to 
      day 100. After exposure to 3Gy of X-rays, the frequencies of non-reciprocal 
      translocations decreased with time, whereas reciprocal translocations between 
      days 7 and 100 were not significantly less frequent than at day 1. A comparison 
      of observed and expected numbers of translocations involving individual 
      chromosomes showed that at day 1 after irradiation, distribution of X-ray-induced 
      translocations among the painted chromosomes was proportional to their DNA 
      content. However, at day 100 after exposure, the observed translocations 
      involving chromosome 2 were more frequent than expected, those involving 
      chromosomes 8 and 15 were less frequent than expected, while chromosomes 1, 6, X 
      and Y were involved as frequently as expected. CONCLUSION: Among induced 
      translocations, non-reciprocal translocations are relatively unstable, especially 
      after exposure to high-dose X-rays. While the initial distribution of 
      X-ray-induced translocations is proportional among the painted chromosomes, the 
      persistence of these translocations is heterogeneous.
FAU - Xiao, Y
AU  - Xiao Y
AD  - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University 
      Medical Centre, The Netherlands.
FAU - Darroudi, F
AU  - Darroudi F
FAU - Grigorova, M
AU  - Grigorova M
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Aneuploidy
MH  - Animals
MH  - Bone Marrow Cells/*radiation effects
MH  - *Chromosome Aberrations
MH  - Female
MH  - Male
MH  - Mice
MH  - Translocation, Genetic
MH  - *Whole-Body Irradiation
MH  - X-Rays
EDAT- 1999/11/30 00:00
MHDA- 1999/11/30 00:01
CRDT- 1999/11/30 00:00
PHST- 1999/11/30 00:00 [pubmed]
PHST- 1999/11/30 00:01 [medline]
PHST- 1999/11/30 00:00 [entrez]
AID - 10.1080/095530099139593 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1999 Sep;75(9):1119-28. doi: 10.1080/095530099139593.