PMID- 10529123
OWN - NLM
STAT- MEDLINE
DCOM- 19991118
LR  - 20171116
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 26
IP  - 10
DP  - 1999 Oct
TI  - Synovium infiltrating T cells induce excessive synovial cell function through 
      CD28/B7 pathway in patients with rheumatoid arthritis.
PG  - 2094-101
AB  - OBJECTIVE: To clarify involvement of synovial T cells in the development of 
      synovial inflammation in patients with rheumatoid arthritis (RA), we analyzed 
      cellular interactions between synovial cells and infiltrating T cells via 
      CD28/B7-1 and B7-2. METHODS: Synovial cells and infiltrating T cells were 
      recovered separately from RA synovial tissues. Expression of CD28, B7-1, and B7-2 
      of synovial cells was analyzed by immunohistochemical staining and 
      immunofluorescence analysis. Interleukin 1beta (IL-1beta), IL-6, and matrix 
      metalloprotease 3 (MMP-3) secreted by synovial cells in the presence of 
      infiltrating T cells were measured by ELISA. Nuclear transcription factor CD28 
      responsive complex was detected by a gel shift assay. RESULTS: Both CD28+ T cells 
      and B7-1/B7-2+ cells were found accumulating in the mononuclear cell infiltrate 
      of RA synovial tissues and B7-1/B7-2+ cells were mainly LeuM3+ synovial cells. 
      CD28 responsive complex was detected in nuclear extracts of freshly isolated 
      lymphocytes from RA synovial tissues, but not those from osteoarthritis synovial 
      tissues or normal peripheral blood, suggesting in vivo activation of T cells by 
      the CD28/B7-1/B7-2 interactions. The irradiated autologous synovium infiltrating 
      T cells notably enhanced IL-1beta, IL-6, and MMP-3 production by the synovial 
      cells. The enhancement of proinflammatory cytokine and MMP-3 production by the 
      synovial cells co-cultured with the T cells was abolished by the addition of 
      CTLA4-Ig, anti-B7-1, and anti-B7-2 monoclonal antibodies. CONCLUSION: These 
      results suggest that cellular interactions between synovium infiltrating T 
      lymphocytes and synovial cells via B7/CD28 pathways are intimately associated 
      with development and exacerbation of inflammation in RA synovial cells.
FAU - Shimoyama, Y
AU  - Shimoyama Y
AD  - Department of Immunology, St. Marianna University School of Medicine, Kawasaki, 
      Kanagawa, Japan.
FAU - Nagafuchi, H
AU  - Nagafuchi H
FAU - Suzuki, N
AU  - Suzuki N
FAU - Ochi, T
AU  - Ochi T
FAU - Sakane, T
AU  - Sakane T
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD28 Antigens)
RN  - 0 (CD86 protein, human)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/immunology/*metabolism
MH  - Arthritis, Rheumatoid/*immunology/metabolism/pathology
MH  - B7-1 Antigen/immunology/*metabolism
MH  - B7-2 Antigen
MH  - CD28 Antigens/immunology/*metabolism
MH  - Cell Communication
MH  - Humans
MH  - Immunohistochemistry
MH  - Membrane Glycoproteins/immunology/*metabolism
MH  - Middle Aged
MH  - Synovial Membrane/*immunology/metabolism/pathology
MH  - T-Lymphocytes/immunology/*metabolism
EDAT- 1999/10/21 00:00
MHDA- 1999/10/21 00:01
CRDT- 1999/10/21 00:00
PHST- 1999/10/21 00:00 [pubmed]
PHST- 1999/10/21 00:01 [medline]
PHST- 1999/10/21 00:00 [entrez]
PST - ppublish
SO  - J Rheumatol. 1999 Oct;26(10):2094-101.