PMID- 10529376
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 264
IP  - 2
DP  - 1999 Oct 22
TI  - Characterization of the MEN1 ortholog in zebrafish.
PG  - 404-8
AB  - Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant syndrome 
      predisposing to multiple tumors. The responsible gene, MEN1, has been identified 
      and inactivating mutations reported. It encodes a protein named menin, which 
      lacks homology to any known proteins. Comparative genomics is used to ascertain 
      important functional domains via the identification of evolutionary conserved 
      regions. Here we report the sequencing and characterization of the MEN1 gene in 
      zebrafish (Danio rerio) at the cDNA level. Zebrafish menin is a 617 amino acid 
      protein and, when compared with human and rodent proteins, shows 75% and 76% 
      similarity, respectively. The most conserved region is amino acid residues 41-322 
      which shows a human/zebrafish similarity of 83%. Amino acids affected by 
      inactivating missense mutations in MEN1 patients in this region are completely 
      conserved between human and zebrafish. Such high correlation between conservation 
      throughout evolution and mutation position strongly emphasizes the importance of 
      this region.
CI  - Copyright 1999 Academic Press.
FAU - Khodaei, S
AU  - Khodaei S
AD  - Department of Molecular Medicine, CMM L8, Karolinska Hospital, Stockholm, S-171 
      76, Sweden. Khodaei@cmm.ki.se
FAU - O'Brien, K P
AU  - O'Brien KP
FAU - Dumanski, J
AU  - Dumanski J
FAU - Wong, F K
AU  - Wong FK
FAU - Weber, G
AU  - Weber G
LA  - eng
SI  - GENBANK/AJ245952
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA, Complementary)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - Evolution, Molecular
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Neoplasm Proteins/chemistry/*genetics
MH  - *Proto-Oncogene Proteins
MH  - Sequence Alignment
MH  - Zebrafish/*genetics
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
AID - S0006-291X(99)91529-2 [pii]
AID - 10.1006/bbrc.1999.1529 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Oct 22;264(2):404-8. doi: 
      10.1006/bbrc.1999.1529.