PMID- 10530719
OWN - NLM
STAT- MEDLINE
DCOM- 19991119
LR  - 20071115
IS  - 0037-1963 (Print)
IS  - 0037-1963 (Linking)
VI  - 36
IP  - 4
DP  - 1999 Oct
TI  - Molecular approaches to the diagnosis and evaluation of lymphoid malignancies.
PG  - 373-89
AB  - The processes of somatic immunoglobulin (Ig) and T-cell receptor (TCR) gene 
      rearrangement that occur in lymphoid precursors provide insights into the 
      pathogenesis and molecular analysis of lymphoid malignancies, in addition to the 
      more universal molecular oncogenic mechanisms. Detection of lymphoid clonality 
      can help distinguish polyclonal reactive disorders from clonal, predominantly, 
      but not exclusively, malignant proliferations. Ig/TCR V-(D)-J polymerase chain 
      reaction (PCR) amplification has largely replaced Southern blotting, and the 
      techniques of PCR product analysis are evolving rapidly. V-(D)-J errors are often 
      involved in genetic abnormalities leading to lymphoid malignancies, with 
      consequent deregulated expression of the associated proto-oncogenes. Genetic 
      abnormalities producing fusion transcripts and chimeric proteins are also 
      frequent, particularly in acute lymphoblastic leukemia (ALL). A variety of 
      molecular techniques, including reverse-transcriptase (RT)-PCR, Southern 
      blotting, and fluorescence in situ hybridization (FISH) are finding an 
      increasingly established place in the diagnosis, prognostic evaluation, 
      therapeutic stratification, and follow-up of lymphoblastic leukemias, and it is 
      likely that the same methods will be applied to non-Hodgkin's lymphoma (NHL) and 
      to chronic leukemias.
FAU - Macintyre, E A
AU  - Macintyre EA
AD  - Laboratoire d'Hematologie and CNRS UMR 8603, Hopital Necker-Enfants Malades, 
      Universite Paris V 149, France.
FAU - Delabesse, E
AU  - Delabesse E
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Semin Hematol
JT  - Seminars in hematology
JID - 0404514
SB  - IM
MH  - *Cytogenetic Analysis
MH  - Gene Rearrangement/immunology
MH  - Humans
MH  - Leukemia, Lymphoid/diagnosis
MH  - Lymphoma/diagnosis
MH  - Lymphoproliferative Disorders/*diagnosis/genetics/immunology
RF  - 95
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
PST - ppublish
SO  - Semin Hematol. 1999 Oct;36(4):373-89.