PMID- 10531211
OWN - NLM
STAT- MEDLINE
DCOM- 19991116
LR  - 20240407
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 67
IP  - 11
DP  - 1999 Nov
TI  - Temporal sequence of pulmonary and systemic inflammatory responses to graded 
      polymicrobial peritonitis in mice.
PG  - 5642-50
AB  - The lungs are the remote organ most commonly affected in human peritonitis. The 
      major goals of this study were to define the dose- and time-dependent 
      relationship between graded septic peritonitis and systemic and pulmonary 
      inflammatory responses in mice. BALB/c mice were treated with intraperitoneal 
      polymicrobial inoculi and sacrificed at 3, 12, and 24 h. The treatment protocol 
      resulted in distinct groups of animals with respect to mortality rate, kinetics, 
      and concentrations of a broad spectrum of pro- and anti-inflammatory endogenous 
      mediators, intrapulmonary bacterial accumulation, and static lung compliance. In 
      sublethally infected mice, pulmonary bacterial proliferation was controlled. 
      Levels of monocyte chemoattractant protein-1 (MCP-1), interleukin-10, 
      interleukin-6, granulocyte colony-stimulating factor (G-CSF), and tumor necrosis 
      factor (TNF) in plasma were elevated 3 h after infection exclusively. At 3 h, 
      MCP-1, gamma interferon, and TNF were detected in extracts of pulmonary tissue or 
      in bronchoalveolar lavage (BAL) fluid. Static lung compliance (C(st)) was 
      transiently decreased at 12 h. In contrast, in lethally infected mice pulmonary 
      bacterial proliferation was not contained. Concentrations of MCP-1, G-CSF, and 
      TNF in plasma were maximal at 24 h, as were pulmonary MCP-1 levels. Lung 
      myeloperoxidase activity was increased at 3, 12, and 24 h. C(st) was reduced 
      after 3 h and did not reach control values at 24 h. Pulmonary cyclooxygenase-2 
      mRNA and eicosanoids in BAL fluid and plasma were elevated at 3 and 24 h. This 
      study shows that polymicrobial peritonitis in mice leads to dose-dependent 
      systemic and pulmonary inflammation accompanied by a decrease in lung compliance.
FAU - Stamme, C
AU  - Stamme C
AD  - Biochemical Pharmacology, University of Konstanz, University Hospital Hannover, 
      Germany.
FAU - Bundschuh, D S
AU  - Bundschuh DS
FAU - Hartung, T
AU  - Hartung T
FAU - Gebert, U
AU  - Gebert U
FAU - Wollin, L
AU  - Wollin L
FAU - Nusing, R
AU  - Nusing R
FAU - Wendel, A
AU  - Wendel A
FAU - Uhlig, S
AU  - Uhlig S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Cytokines)
RN  - 0 (Eicosanoids)
RN  - 0 (Proteins)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - Bacterial Infections/*immunology/physiopathology
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Cytokines/blood
MH  - Eicosanoids/blood
MH  - Gene Expression
MH  - Inflammation/*immunology/physiopathology
MH  - Lung Compliance
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peritonitis/*immunology/physiopathology
MH  - Peroxidase/metabolism
MH  - Pneumonia/*immunology/physiopathology
MH  - Proteins/analysis
PMC - PMC96937
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
PMCR- 1999/11/01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
PHST- 1999/11/01 00:00 [pmc-release]
AID - 0436 [pii]
AID - 10.1128/IAI.67.11.5642-5650.1999 [doi]
PST - ppublish
SO  - Infect Immun. 1999 Nov;67(11):5642-50. doi: 10.1128/IAI.67.11.5642-5650.1999.