PMID- 10532970
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20190905
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 84
IP  - 7
DP  - 1999 Nov
TI  - Chromosomal aberrations in transitional cell carcinoma that are predictive of 
      disease outcome are independent of polyploidy.
PG  - 775-9
AB  - OBJECTIVE: To determine whether aneusomy for chromosomes 7, 9 and 17 (reported to 
      predict recurrence in up to 65% of patients with superficial transitional cell 
      bladder cancer and thus providing the opportunity for early and effective 
      treatment) reflects specific genetic events on these chromosomes or merely wider 
      unspecific genetic damage to the cell, e.g. that increased copy numbers for 7 and 
      17 reflect tumour polyploidy. MATERIALS AND METHODS: The study comprised 25 
      primary tumours; 6 microm thick sections from formalin-fixed and 
      paraffin-embedded tumours were analysed. Chromosome copy numbers were determined 
      by fluorescence in situ hybridization (FISH) using pericentromeric probes for 
      chromosomes 7, 8, 9, 10, 11 and 17. A minimum of 200 nuclei per tumour area were 
      scored by two independent observers. RESULTS: Eight of the 25 tumours examined 
      (32%) showed no evidence of chromosomal abnormalities as detected by FISH for any 
      chromosomes analysed. Twelve tumours (48%) showed abnormalities for one or two 
      chromosomes, five tumours (20%) showed abnormalities for multiple chromosomes and 
      one tumour showed abnormalities for all chromosomes analysed, suggestive of 
      polyploidy. CONCLUSIONS: Chromosomal abnormalities predictive of recurrence occur 
      largely in the absence of other gross chromosomal lesions. In a small proportion 
      of cases other chromosomes are affected, but this is almost always distinct from 
      tumour polyploidy.
FAU - Bartlett, J M
AU  - Bartlett JM
AD  - University Department of Surgery, Glasgow Royal Infirmary, Scotland, UK. 
      gqva20@udcf.gla.ac.uk
FAU - Adie, L
AU  - Adie L
FAU - Watters, A D
AU  - Watters AD
FAU - Going, J J
AU  - Going JJ
FAU - Grigor, K M
AU  - Grigor KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
SB  - IM
MH  - Carcinoma, Transitional Cell/*genetics
MH  - Chromosome Aberrations/*genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 7/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Neoplasm Recurrence, Local/genetics
MH  - Neoplasm Staging/methods
MH  - *Polyploidy
MH  - Prognosis
MH  - Retrospective Studies
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 1999/10/26 00:00
MHDA- 1999/10/26 00:01
CRDT- 1999/10/26 00:00
PHST- 1999/10/26 00:00 [pubmed]
PHST- 1999/10/26 00:01 [medline]
PHST- 1999/10/26 00:00 [entrez]
AID - bju268 [pii]
AID - 10.1046/j.1464-410x.1999.00268.x [doi]
PST - ppublish
SO  - BJU Int. 1999 Nov;84(7):775-9. doi: 10.1046/j.1464-410x.1999.00268.x.