PMID- 10534739
OWN - NLM
STAT- MEDLINE
DCOM- 20000112
LR  - 20190831
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 59
IP  - 4
DP  - 1999 Dec
TI  - Estimating the time of HTLV-I infection following mother-to-child transmission in 
      a breast-feeding population in Jamaica.
PG  - 541-6
AB  - Mother-to-child transmission of human T-cell lymphotropic virus type I (HTLV-I) 
      is primarily due to prolonged breast-feeding (>6 months) in the postnatal period. 
      Most infant infections are not identifiable until 12 to 18 months of age by 
      available whole virus Western blot serologic tests because of their inability to 
      distinguish passively transferred maternal antibody from infant antibody. We 
      investigated two methods to assess more accurately the time of infant infection. 
      In prospectively collected serial biospecimens, HTLV-I-specific immunoglobulin 
      (Ig) isotypes of IgM and IgA were determined by Western blot and HTLV-I proviral 
      DNA was detected by polymerase chain reaction (PCR). IgA and IgG reactivity was 
      assessed in periodic serum samples from 16 HTLV-I-seropositive children while IgM 
      reactivity was assessed in 9 of the 16 children. Approximately three to five 
      samples were tested for each child. IgG reactivity was observed in 100% of 
      children at 24 months of age and 73% of children at 6-12 months of age; however, 
      this could represent maternal and not infant antibody. Both IgA and IgM 
      reactivity were insensitive indicators of infection, with only 50% of children 
      showing reactivity at 24 months of age. PCR testing was performed in biospecimens 
      obtained from 11 of these children. An estimated median time of infection of 11.9 
      months was determined by PCR, which was similar to the median time to infection 
      determined by whole virus Western blot (12.4 months; P = 0.72). PCR tests support 
      a median time to infection that is similar to that estimated by whole virus 
      Western blot.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Furnia, A
AU  - Furnia A
AD  - George Washington University, School of Public Health and Health Services, 
      Washington, DC, USA.
FAU - Lal, R
AU  - Lal R
FAU - Maloney, E
AU  - Maloney E
FAU - Wiktor, S
AU  - Wiktor S
FAU - Pate, E
AU  - Pate E
FAU - Rudolph, D
AU  - Rudolph D
FAU - Waters, D
AU  - Waters D
FAU - Blattner, W
AU  - Blattner W
FAU - Manns, A
AU  - Manns A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Adult
MH  - *Breast Feeding
MH  - Child, Preschool
MH  - DNA, Viral/analysis
MH  - Evaluation Studies as Topic
MH  - Female
MH  - HTLV-I Antibodies/blood
MH  - HTLV-I Infections/immunology/*transmission/virology
MH  - Human T-lymphotropic virus 1/*immunology/isolation & purification
MH  - Humans
MH  - Immunoglobulin A/blood
MH  - Immunoglobulin M/blood
MH  - Infant
MH  - Infant, Newborn
MH  - *Infectious Disease Transmission, Vertical
MH  - Jamaica
MH  - Polymerase Chain Reaction/methods
MH  - Prospective Studies
MH  - Proviruses
MH  - Time Factors
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - 10.1002/(SICI)1096-9071(199912)59:4<541::AID-JMV19>3.0.CO;2-S [pii]
AID - 10.1002/(sici)1096-9071(199912)59:4<541::aid-jmv19>3.0.co;2-s [doi]
PST - ppublish
SO  - J Med Virol. 1999 Dec;59(4):541-6. doi: 
      10.1002/(sici)1096-9071(199912)59:4<541::aid-jmv19>3.0.co;2-s.