PMID- 10535812
OWN - NLM
STAT- MEDLINE
DCOM- 19991222
LR  - 20190815
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 20
IP  - 21
DP  - 1999 Nov
TI  - Guided tissue fabrication from periosteum using preformed biodegradable polymer 
      scaffolds.
PG  - 2007-18
AB  - A successful tissue engineering method for bone replacement would imitate natural 
      bone graft by providing the essential elements for new bone formation using 
      synthetic scaffolds, osteogenic cell populations, and bone induction factors. 
      This is a study of the suitability of various formulations of 
      poly(DL-lactic-co-glycolic acid) (PLGA) foams to provide a tissue conducting 
      scaffold in an ovine model for bone flap fabrication. Three formulations were 
      used of different copolymer ratio and molecular weight. Porous wafers of PLGA 
      were stacked into rectangular chambers (volume 4 cm3) enclosed on five sides. 
      Some chambers also contained autologous morcellized bone graft (MBG). The 
      chambers were inserted with the open face adjacent to the cambium layer of the 
      periosteum in rib beds of seven sheep and harvested after 8 weeks in vivo. Gross 
      and histologic examination of the resulting tissue specimens demonstrated molded 
      units of vascularized tissue generally conforming to the shape of the chambers 
      and firmly attached to the periosteum. Polymer degradation appeared to occur by 
      varying degrees based on polymer formulation. New bone formation was observed 
      only in areas containing MBG. There was no evidence of significant inflammatory 
      reaction or local tissue damage at 8 weeks. We conclude that a PLGA foam scaffold 
      is (1) an efficient conductor of new tissue growth but not osteoinductive, (2) 
      contributes to the shape of molded tissue, and (3) biocompatible when used in 
      this model. Further studies are warranted to develop practical methods to deliver 
      bone induction factors to the system to promote osseous tissue generation 
      throughout the synthetic scaffold.
FAU - Thomson, R C
AU  - Thomson RC
AD  - lnstitute of Biosciences and Bioengineering, Rice University, Houston, TX, USA.
FAU - Mikos, A G
AU  - Mikos AG
FAU - Beahm, E
AU  - Beahm E
FAU - Lemon, J C
AU  - Lemon JC
FAU - Satterfield, W C
AU  - Satterfield WC
FAU - Aufdemorte, T B
AU  - Aufdemorte TB
FAU - Miller, M J
AU  - Miller MJ
LA  - eng
GR  - CA-1667/CA/NCI NIH HHS/United States
GR  - R29-AR42639/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - *Absorbable Implants
MH  - Animals
MH  - *Biocompatible Materials
MH  - Bone Transplantation
MH  - Diffusion Chambers, Culture
MH  - *Guided Tissue Regeneration
MH  - *Implants, Experimental
MH  - *Lactic Acid
MH  - Periosteum/*growth & development
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers
MH  - Polymethyl Methacrylate
MH  - Sheep
OTO - NASA
OT  - Non-programmatic
EDAT- 1999/10/27 00:00
MHDA- 1999/10/27 00:01
CRDT- 1999/10/27 00:00
PHST- 1999/10/27 00:00 [pubmed]
PHST- 1999/10/27 00:01 [medline]
PHST- 1999/10/27 00:00 [entrez]
AID - S0142-9612(99)00103-9 [pii]
AID - 10.1016/s0142-9612(99)00103-9 [doi]
PST - ppublish
SO  - Biomaterials. 1999 Nov;20(21):2007-18. doi: 10.1016/s0142-9612(99)00103-9.