PMID- 10536257
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 844
IP  - 1-2
DP  - 1999 Oct 9
TI  - Treatment with estrogen and progesterone affects relative levels of brain-derived 
      neurotrophic factor mRNA and protein in different regions of the adult rat brain.
PG  - 20-7
AB  - Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and 
      enzyme-linked immunosorbent assay (ELISA) were used to examine the effects of 
      acute estrogen and progesterone replacement on relative levels of brain-derived 
      neurotrophic factor (BDNF) mRNA and protein in different regions of the adult rat 
      brain. Adult ovariectomized animals were killed 53 h after receiving estrogen 
      (E53), 53 h after receiving estrogen and 5 h after receiving progesterone (E53P), 
      or 72 h after receiving estrogen and 24 h after receiving progesterone (E72P). 
      Ovariectomized controls were killed 53 and 72 h after receiving vehicle. Tissues 
      from the hippocampus, pyriform cortex, olfactory bulbs, septum, and nucleus 
      basalis/ventral pallidum were dissected. Tissues from the right hemisphere were 
      processed for quantitative RT-PCR analysis of BDNF mRNA, and tissues from the 
      left hemisphere were processed for the detection and quantification of BDNF 
      protein by ELISA. The results demonstrate significant increases in BDNF mRNA in 
      the pyriform cortex of E53- and E53P-treated animals, as well as an increase in 
      BDNF protein in the pyriform cortex of E72P-treated animals, relative to 
      controls. Significant increases in BDNF mRNA were likewise detected in the 
      hippocampus of E53- and E72P-treated animals, but were accompanied by a 
      significant decrease in BDNF protein in the hippocampus of E53P- and E72P-treated 
      animals relative to controls. No significant changes in BDNF mRNA or protein were 
      detected in the olfactory bulbs, frontal cortex, or nucleus basalis/ventral 
      pallidum following hormone treatment; however, an increase in BDNF protein was 
      detected in the septum of E53-treated animals. This may indicate an increase in 
      the retrograde transport of BDNF from the hippocampus to the septum, which could 
      help account for the decrease in BDNF protein detected in the hippocampus 
      following hormone treatment. These findings demonstrate that hormone replacement 
      significantly affects relative levels of BDNF mRNA and protein within specific 
      regions of the brain. These effects may, in turn, contribute to the effects of 
      estrogen replacement on hippocampal connectivity and cognitive processes that 
      have recently been reported.
FAU - Gibbs, R B
AU  - Gibbs RB
AD  - Department of Pharmaceutical Sciences, University of Pittsburgh School of 
      Pharmacy, 1004 Salk Hall, Pittsburgh, PA 15261, USA. gibbsr@vms.cis.pitt.edu
LA  - eng
GR  - NS28896/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Brain Chemistry/*drug effects
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics
MH  - DNA Primers
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Frontal Lobe/chemistry
MH  - Gene Expression/drug effects
MH  - Hippocampus/chemistry
MH  - Olfactory Bulb/chemistry
MH  - Ovariectomy
MH  - Progesterone/*pharmacology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Septum of Brain/chemistry
EDAT- 1999/10/28 00:00
MHDA- 1999/10/28 00:01
CRDT- 1999/10/28 00:00
PHST- 1999/10/28 00:00 [pubmed]
PHST- 1999/10/28 00:01 [medline]
PHST- 1999/10/28 00:00 [entrez]
AID - S0006-8993(99)01880-6 [pii]
AID - 10.1016/s0006-8993(99)01880-6 [doi]
PST - ppublish
SO  - Brain Res. 1999 Oct 9;844(1-2):20-7. doi: 10.1016/s0006-8993(99)01880-6.