PMID- 10540014
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20190724
IS  - 0022-510X (Print)
IS  - 0022-510X (Linking)
VI  - 169
IP  - 1-2
DP  - 1999 Oct 31
TI  - A receptor for advanced glycosylation endproducts (AGEs) is colocalized with 
      neurofilament-bound AGEs and SOD1 in motoneurons of ALS: immunohistochemical 
      study.
PG  - 87-92
AB  - Neurofilament (NF)-bound AGEs colocalize immunochemically with SOD1 in the 
      motoneurons of patients with ALS. Among three types of AGE receptors reported in 
      the human brain, AGE-R1 (oligosaccharyltransferase family) and AGE-R2 (substrate 
      of protein kinase C) have been found in neurons, while AGE-R3 is restricted to 
      glia. The present study investigates which of these receptors may be responsible 
      for binding AGEs in the NF conglomerates of motoneurons. Immunostaining of 
      paraffin sections from eight ALS patients (five sporadic and three familial) and 
      three control cases was performed with antibodies directed against R1 and R2, in 
      parallel with those against AGEs and SOD1. The sites of AGE-R1 immunoreactivity 
      (IR) in motoneurons were in conformity to those of NF-associated AGE and SOD1 
      IRs. By contrast, the IR of R2 was negative in NF conglomerates. Negative R2 IR 
      for NF conglomerates was outlined by surrounding coarse R2 immunopositive 
      granules in the perikaryon. No IR for R1 or R2 was found in hyaline or Bunina 
      inclusions. There was no extraneuronal expression of IR for AGE-R1 or AGEs in 
      microglia or astroglia around the NF accumulation. The colocalization of AGE, 
      AGE-R1, and SOD1 at NF conglomerates in motoneurons supports the notion that 
      AGE-mediated oxidative stress and protein aggregation may be implicated in NF 
      conglomeration and ALS pathogenesis.
FAU - Chou, S M
AU  - Chou SM
AD  - Norris ALS/MDA Research Center, California Pacific Medical Center, San Francisco, 
      CA 94116, USA.
FAU - Han, C Y
AU  - Han CY
FAU - Wang, H S
AU  - Wang HS
FAU - Vlassara, H
AU  - Vlassara H
FAU - Bucala, R
AU  - Bucala R
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Neurol Sci
JT  - Journal of the neurological sciences
JID - 0375403
RN  - 0 (Receptor for Advanced Glycation End Products)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (SOD1 protein, human)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.15.1.1 (Superoxide Dismutase-1)
SB  - IM
MH  - *Amyotrophic Lateral Sclerosis
MH  - Humans
MH  - Immunohistochemistry
MH  - Motor Neurons/*chemistry
MH  - Receptor for Advanced Glycation End Products
MH  - Receptors, Immunologic/*analysis
MH  - Superoxide Dismutase/*analysis
MH  - Superoxide Dismutase-1
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - S0022-510X(99)00222-1 [pii]
AID - 10.1016/s0022-510x(99)00222-1 [doi]
PST - ppublish
SO  - J Neurol Sci. 1999 Oct 31;169(1-2):87-92. doi: 10.1016/s0022-510x(99)00222-1.