PMID- 10542131
OWN - NLM
STAT- MEDLINE
DCOM- 20000829
LR  - 20091119
IS  - 1522-4724 (Print)
IS  - 1522-4724 (Linking)
VI  - 2
IP  - 2
DP  - 1999 Aug
TI  - Cloning and expression of a murine histone deacetylase 3 (mHdac3) cDNA and 
      mapping to a region of conserved synteny between murine chromosome 18 and human 
      chromosome 5.
PG  - 91-6
AB  - Histone deacetylases (HDACs) are enzymes that play a pivotal role in 
      transcription, differentiation, and cell cycle progression. We previously cloned 
      human HDAC3 cDNA and showed that its transfection into THP-1 cells results in 
      G2/M cell cycle accumulation. Using bioinformatic screening and PCR, we have now 
      cloned the murine Hdac3 cDNA, which encodes a 428-amino-acid protein with near 
      complete identity to its human ortholog. To establish a link to a potential 
      disease locus, we performed PCR-based chromosomal mapping for the mHdac3 gene and 
      chromosomal fluorescence in situ hybridization (FISH) for the human gene. mHdac3 
      localizes to chromosome 18 and human HDAC3 gene localizes to a syntenic region in 
      chromosome 5 at band q31.3-q32 telomeric to the cytokine gene cluster. 
      Transfection of mHdac3 into HeLa cells led to accumulation in G2/M. Our results 
      suggest a cell cycle function for murine Hdac3, reflecting the complex regulatory 
      roles of this gene family.
FAU - Dangond, F
AU  - Dangond F
AD  - Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, 
      Boston, Massachusetts 02115, USA. dangond@cnd.bwh.harvard.edu
FAU - Foerznler, D
AU  - Foerznler D
FAU - Weremowicz, S
AU  - Weremowicz S
FAU - Morton, C C
AU  - Morton CC
FAU - Beier, D R
AU  - Beier DR
FAU - Gullans, S R
AU  - Gullans SR
LA  - eng
SI  - GENBANK/AF098295
GR  - DK36031/DK/NIDDK NIH HHS/United States
GR  - HD29028/HD/NICHD NIH HHS/United States
GR  - HG00951/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol Res Commun
JT  - Molecular cell biology research communications : MCBRC
JID - 100889076
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - EC 3.5.1.98 (histone deacetylase 3)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Cycle/genetics
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 5
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - HeLa Cells
MH  - Histone Deacetylases/*genetics/*metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney/cytology/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - Transfection
EDAT- 1999/11/05 08:00
MHDA- 2000/09/02 11:01
CRDT- 1999/11/05 08:00
PHST- 1999/11/05 08:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 1999/11/05 08:00 [entrez]
AID - S1522472499901563 [pii]
AID - 10.1006/mcbr.1999.0156 [doi]
PST - ppublish
SO  - Mol Cell Biol Res Commun. 1999 Aug;2(2):91-6. doi: 10.1006/mcbr.1999.0156.