PMID- 10542218
OWN - NLM
STAT- MEDLINE
DCOM- 19991213
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 45
DP  - 1999 Nov 5
TI  - trans-Retinoic acid blocks platelet-derived growth factor-BB-induced expression 
      of the murine monocyte chemoattractant-1 gene by blocking the assembly of a 
      promoter proximal Sp1 binding site.
PG  - 31909-16
AB  - Proper regulation of the CC chemokine MCP-1 (monocyte chemoattractant protein-1) 
      is important for normal inflammatory responses. MCP-1 is regulated by a wide 
      variety of agents, including platelet-derived growth factor-BB (PDGF-BB) and 
      tumor necrosis factor-alpha (TNF). Using both in vivo and in vitro assays, the 
      elements required for expression between these two cytokines were compared. In 
      vivo genomic footprinting showed that PDGF-BB induction occurred through the 
      occupancy of the proximal regulatory region, and unlike TNF induction, no changes 
      in the NF-kappaB binding, distal regulatory region occurred. Treatment of cells 
      with trans-retinoic acid, an inhibitor of PDGF-BB activity, resulted in a 50% 
      reduction in PDGF-BB-mediated induction and a concomitant block in the assembly 
      of the proximal regulatory region. trans-Retinoic acid had minimal effect on TNF 
      induction or promoter occupancy. An inhibitor of histone deacetylation was found 
      to stimulate expression of MCP-1 in a manner that correlated with increased 
      accessibility to the proximal regulatory region. These results show that the 
      mechanisms of PDGF-BB and TNF activation of MCP-1 are distinct, although they 
      both require the proximal regulatory region Sp1 binding site. The results also 
      suggest that part of the mechanism used by both of these cytokines involves a 
      process that regulates transcription factor access to the regulatory regions.
FAU - Ping, D
AU  - Ping D
AD  - Department of Microbiology, Emory University School of Medicine, Atlanta, Georgia 
      30322, USA.
FAU - Boekhoudt, G
AU  - Boekhoudt G
FAU - Boss, J M
AU  - Boss JM
LA  - eng
GR  - CA74271/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 148710-94-5 (Sp3 Transcription Factor)
RN  - 1B56C968OA (Becaplermin)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Becaplermin
MH  - Binding Sites
MH  - Chemokine CCL2/*genetics
MH  - DNA-Binding Proteins/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fibroblasts/drug effects/metabolism
MH  - Mice
MH  - Platelet-Derived Growth Factor/*pharmacology
MH  - *Promoter Regions, Genetic
MH  - Proto-Oncogene Proteins c-sis
MH  - Sp1 Transcription Factor/*metabolism
MH  - Sp3 Transcription Factor
MH  - Transcription Factors/metabolism
MH  - Tretinoin/*pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Zinc Fingers
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S0021-9258(19)51506-X [pii]
AID - 10.1074/jbc.274.45.31909 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 5;274(45):31909-16. doi: 10.1074/jbc.274.45.31909.