PMID- 10543482
OWN - NLM
STAT- MEDLINE
DCOM- 19991109
LR  - 20190628
IS  - 0003-4975 (Print)
IS  - 0003-4975 (Linking)
VI  - 68
IP  - 4
DP  - 1999 Oct
TI  - Phenylephrine induces delayed cardioprotection against necrosis without 
      amelioration of stunning.
PG  - 1219-24
AB  - BACKGROUND: Alpha-adrenergic stimulation induces protection in reperfused 
      ischemic (I/R) myocardium 24 hours later. We tested the hypothesis that 
      phenylephrine improves dysfunction after global I/R by limiting cell death not 
      stunning. METHODS: Rabbits were pretreated with either phenylephrine or vehicle. 
      Twenty-four hours later, isolated hearts underwent either 45 (infarction 
      protocol) or 20 minutes (stunning protocol) of global ischemia before 2 hours of 
      reperfusion (n = 6 per group). Cell death was determined by triphenyl tetrazolium 
      chloride staining (infarction) and terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL) (apoptosis). RESULTS: Compared with vehicle, 
      phenylephrine pretreatment improved post-I/R-developed pressures in hearts after 
      infarction (53.2 +/- 4.0 vs 35.8 +/- 4.1 mm Hg, p = 0.01) but not stunning 
      protocol (64.3 +/- 8.9 vs 57.7 +/- 6.2 mm Hg, p = NS). The improved developed 
      pressure was due to better diastolic recovery. Systolic pressures were similar 
      between groups. Phenylephrine markedly decreased infarction (9.0 +/- 1.9% vs 40.8 
      +/- 1.8% for vehicle, p < 0.001) and TUNEL-positive staining. Stunned hearts of 
      either group had less than 3% infarction and no apoptosis. CONCLUSIONS: 
      Phenylephrine pretreatment 24 hours before global I/R improves function by 
      limiting infarction but not stunning.
FAU - Baghelai, K
AU  - Baghelai K
AD  - Department of Surgery, Medical College of Virginia, Virginia Commonwealth 
      University, Richmond 23298, USA. kbaghela@hsc.vcu.edu
FAU - Graham, L J
AU  - Graham LJ
FAU - Wechsler, A S
AU  - Wechsler AS
FAU - Jakoi, E R
AU  - Jakoi ER
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Ann Thorac Surg
JT  - The Annals of thoracic surgery
JID - 15030100R
RN  - 0 (Cardiotonic Agents)
RN  - 1WS297W6MV (Phenylephrine)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/physiology
MH  - Cardiotonic Agents/*pharmacology
MH  - Cell Death/drug effects/physiology
MH  - Female
MH  - Male
MH  - Myocardial Contraction/drug effects/physiology
MH  - Myocardial Reperfusion Injury/pathology/*physiopathology
MH  - Myocardial Stunning/pathology/*physiopathology
MH  - Myocardium/*pathology
MH  - Necrosis
MH  - Phenylephrine/*pharmacology
MH  - Rabbits
EDAT- 1999/10/30 00:00
MHDA- 1999/10/30 00:01
CRDT- 1999/10/30 00:00
PHST- 1999/10/30 00:00 [pubmed]
PHST- 1999/10/30 00:01 [medline]
PHST- 1999/10/30 00:00 [entrez]
AID - S0003-4975(99)00979-0 [pii]
AID - 10.1016/s0003-4975(99)00979-0 [doi]
PST - ppublish
SO  - Ann Thorac Surg. 1999 Oct;68(4):1219-24. doi: 10.1016/s0003-4975(99)00979-0.