PMID- 10543820
OWN - NLM
STAT- MEDLINE
DCOM- 19991213
LR  - 20200724
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 65
IP  - 11
DP  - 1999 Nov
TI  - Oxidation of methyl halides by the facultative methylotroph strain IMB-1.
PG  - 5035-41
AB  - Washed cell suspensions of the facultative methylotroph strain IMB-1 grown on 
      methyl bromide (MeBr) were able to consume methyl chloride (MeCl) and methyl 
      iodide (MeI) as well as MeBr. Consumption of >100 microM MeBr by cells grown on 
      glucose, acetate, or monomethylamine required induction. Induction was inhibited 
      by chloramphenicol. However, cells had a constitutive ability to consume low 
      concentrations (<20 nM) of MeBr. Glucose-grown cells were able to readily oxidize 
      [(14)C]formaldehyde to (14)CO(2) but had only a small capacity for oxidation of 
      [(14)C]methanol. Preincubation of cells with MeBr did not affect either activity, 
      but MeBr-induced cells had a greater capacity for [(14)C]MeBr oxidation than did 
      cells without preincubation. Consumption of MeBr was inhibited by MeI, and MeCl 
      consumption was inhibited by MeBr. No inhibition of MeBr consumption occurred 
      with methyl fluoride, propyl iodide, dibromomethane, dichloromethane, or 
      difluoromethane, and in addition cells did not oxidize any of these compounds. 
      Cells displayed Michaelis-Menten kinetics for the various methyl halides, with 
      apparent K(s) values of 190, 280, and 6,100 nM for MeBr, MeI, and MeCl, 
      respectively. These results suggest the presence of a single oxidation enzyme 
      system specific for methyl halides (other than methyl fluoride) which runs 
      through formaldehyde to CO(2). The ease of induction of methyl halide oxidation 
      in strain IMB-1 should facilitate its mass culture for the purpose of reducing 
      MeBr emissions to the atmosphere from fumigated soils.
FAU - Schaefer, J K
AU  - Schaefer JK
AD  - U.S. Geological Survey, Menlo Park, California 94025, USA.
FAU - Oremland, R S
AU  - Oremland RS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Acetates)
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Hydrocarbons, Iodinated)
RN  - 0 (Methylamines)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 66974FR9Q1 (Chloramphenicol)
RN  - 9V42E1Z7B6 (methyl bromide)
RN  - A6R43525YO (Methyl Chloride)
RN  - BSF23SJ79E (methylamine)
RN  - DAT010ZJSR (methyl iodide)
RN  - IY9XDZ35W2 (Glucose)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Acetates/metabolism
MH  - Alphaproteobacteria/drug effects/growth & development/*metabolism
MH  - Carbon Radioisotopes
MH  - Chloramphenicol/pharmacology
MH  - Formaldehyde/metabolism
MH  - Glucose/metabolism
MH  - Hydrocarbons, Brominated/metabolism
MH  - Hydrocarbons, Iodinated/*metabolism
MH  - Kinetics
MH  - Methanol/metabolism
MH  - Methyl Chloride/*metabolism
MH  - Methylamines/metabolism
MH  - Oxidation-Reduction
MH  - Soil Microbiology
PMC - PMC91678
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
PMCR- 1999/11/01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
PHST- 1999/11/01 00:00 [pmc-release]
AID - 0701 [pii]
AID - 10.1128/AEM.65.11.5035-5041.1999 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1999 Nov;65(11):5035-41. doi: 
      10.1128/AEM.65.11.5035-5041.1999.