PMID- 10544156
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20181113
IS  - 0091-6765 (Print)
IS  - 0091-6765 (Linking)
VI  - 107
IP  - 11
DP  - 1999 Nov
TI  - Expression of the constitutive and inducible forms of heat shock protein 70 in 
      human proximal tubule cells exposed to heat, sodium arsenite, and CdCl(2).
PG  - 887-93
AB  - We determined the expression of the constitutive (hsc 70) and inducible (hsp 70) 
      forms of heat shock protein 70 mRNA and protein in human proximal tubule (HPT) 
      cells exposed to lethal and sublethal concentrations of Cd(+2) under both acute 
      and extended conditions of exposure. The HPT cells exhibited the classic heat 
      shock response when subjected to a physical (heat) or chemical stress (sodium 
      arsenite); hsc 70 mRNA and protein levels were constant or slightly increased, 
      whereas hsp 70 mRNA and protein were greatly elevated. Acute exposure to 53.4 
      microM CdCl(2) for 4 hr failed to increase either hsc 70 mRNA or protein, a 
      finding similar to that observed under classic conditions of stress. However, 
      under identical conditions of acute exposure to Cd(2+), the expected increase in 
      hsp 70 protein level was suppressed as compared to that found under classic 
      conditions of physical or chemical stress. The decrease in hsp 70 protein level 
      correlated to the reduced expression of mRNA from the hsp 70B gene. The 
      expression of mRNA from the hsp 70A and hsp 70C genes was similar to that found 
      when the cells were treated with heat shock or sodium arsenite. We modeled an 
      extended exposure to Cd(2+) by treating the cells continuously with Cd(2+) at 
      both lethal and sublethal levels over a 16-day time course. Chronic exposure to 
      Cd(2+) failed to increase either hsc 70 mRNA or protein levels in the HPT cells 
      at a nonlethal dosage level and decreased hsc 70 mRNA and protein levels late in 
      the time course of lethal exposure. Under identical conditions, the expression of 
      hsp 70 protein remained at basal levels that were only marginally detectable 
      throughout the time course. Hsp 70A and hsp 70C mRNA levels were unaltered by 
      extended exposure to Cd(2+), and hsp 70B mRNA was not detected during the 16-day 
      time course. Cd(2+) is a poor inducer of hsc 70 and hsp 70 in the proximal tubule 
      under both acute and long-term exposure. These results reinforce the fact that 
      the expression of hsp 70 protein does not result from the transcription of a 
      single gene, but is derived from what may be a complex interplay of several 
      underlying genes.
FAU - Somji, S
AU  - Somji S
AD  - Robert C. Byrd Health Sciences Center, Department of Pathology, West Virginia 
      University, Morgantown, West Virginia, USA.
FAU - Todd, J H
AU  - Todd JH
FAU - Sens, M A
AU  - Sens MA
FAU - Garrett, S H
AU  - Garrett SH
FAU - Sens, D A
AU  - Sens DA
LA  - eng
GR  - ES07687/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Arsenites)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sodium Compounds)
RN  - 48OVY2OC72 (sodium arsenite)
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Arsenites/*adverse effects/pharmacology
MH  - Cadmium Chloride/*adverse effects/pharmacology
MH  - Cell Culture Techniques
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/*adverse effects/pharmacology
MH  - HSP70 Heat-Shock Proteins/*biosynthesis/genetics
MH  - Hot Temperature
MH  - Humans
MH  - Kidney Tubules, Proximal/*drug effects/physiology
MH  - RNA, Messenger/biosynthesis/drug effects
MH  - Sodium Compounds/*adverse effects/pharmacology
MH  - Transcription, Genetic
PMC - PMC1566690
EDAT- 1999/11/02 00:00
MHDA- 1999/11/02 00:01
PMCR- 1999/11/01
CRDT- 1999/11/02 00:00
PHST- 1999/11/02 00:00 [pubmed]
PHST- 1999/11/02 00:01 [medline]
PHST- 1999/11/02 00:00 [entrez]
PHST- 1999/11/01 00:00 [pmc-release]
AID - sc271_5_1835 [pii]
AID - 10.1289/ehp.99107887 [doi]
PST - ppublish
SO  - Environ Health Perspect. 1999 Nov;107(11):887-93. doi: 10.1289/ehp.99107887.