PMID- 10547157
OWN - NLM
STAT- MEDLINE
DCOM- 19991208
LR  - 20081121
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 19
IP  - 10
DP  - 1999 Oct
TI  - Cloning of a cDNA encoding bovine interleukin-18 and analysis of IL-18 expression 
      in macrophages and its IFN-gamma-inducing activity.
PG  - 1169-77
AB  - Interleukin-18 (IL-18) is a recently described cytokine that enhances 
      interferon-gamma (IFN-gamma) production, either independently or synergistically 
      with IL-12. These properties identify IL-18 as an immunoregulatory cytokine that 
      may be pivotal in host defense against intracellular pathogens. We have isolated 
      and sequenced a cDNA encoding bovine IL-18. The open reading frame (ORF) is 582 
      bp in length, encoding a predicted 192 amino acid (aa) precursor protein. 
      Multiple sequence alignment demonstrated that bovine IL-18 has 65% and 78% 
      identity with the predicted amino acid sequences of murine and human IL-18, 
      respectively. IL-18 mRNA was constitutively present in bovine peripheral blood 
      monocyte-derived macrophages (MDM), with no upregulation on stimulation with 
      lipopolysaccharide (LPS). IL-18 transcripts were weakly detected in B lymphocytes 
      but inducible in the B cell line BL-3. Human recombinant IL-18 (rHuIL-18) induced 
      IFN-gamma production by PHA-stimulated peripheral blood mononuclear cells (PBMC), 
      which was potentiated by rHuIL-12. Further, rHuIL-12 and rHuIL-18 enhanced 
      proliferation of untreated PBMC. Antigen-specific T cell lines demonstrated 
      IL-18-dependent enhancement of IFN-gamma production, indicating that bovine T 
      cells are one of the leukocyte subsets that respond to IL-18. Analysis of IL-18 
      expression and its ability to induce IFN-gamma production by bovine lymphocytes 
      are important considerations for understanding mechanisms of protective immunity 
      and designing vaccines for intracellular pathogens.
FAU - Shoda, L K
AU  - Shoda LK
AD  - Department of Veterinary Microbiology and Pathology, Washington State University, 
      Pullman 99164-7040, USA.
FAU - Zarlenga, D S
AU  - Zarlenga DS
FAU - Hirano, A
AU  - Hirano A
FAU - Brown, W C
AU  - Brown WC
LA  - eng
SI  - GENBANK/AF124789
GR  - AI-30136/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (DNA, Complementary)
RN  - 0 (Interferon Inducers)
RN  - 0 (Interleukin-18)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary/*genetics
MH  - Gene Expression Regulation/*physiology
MH  - Genetic Code
MH  - Humans
MH  - *Interferon Inducers
MH  - Interferon-gamma/*biosynthesis
MH  - Interleukin-18/*genetics
MH  - Leukocytes, Mononuclear/metabolism
MH  - Macrophages/*metabolism
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - T-Lymphocyte Subsets/metabolism
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - 10.1089/107999099313118 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 1999 Oct;19(10):1169-77. doi: 10.1089/107999099313118.