PMID- 10547276
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20131121
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 11
IP  - 11
DP  - 1999 Nov
TI  - Increased monocyte MCP-1 production in acute alcoholic hepatitis.
PG  - 875-81
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a potent mononuclear cell-specific 
      chemotactic protein. MCP-1 is a candidate chemoattractant for activation and 
      hepatic infiltration of mononuclear cells in alcoholic hepatitis (AH). Blood was 
      collected from 15 patients with AH (mean bilirubin 17.6+/-3.5 mg/dl; normal 0. 
      2-1.0 mg/dl) on admission and at time points for up to 6 months. Peripheral blood 
      monocytes were isolated and MCP-1 production assessed by measuring MCP-1 
      concentrations in monocyte culture supernatants after overnight (20 h) 
      incubation. Monocytes from normal subjects did not product detectable MCP-1 
      unless stimulated with endotoxin (LPS;5 microg/ml). The mean level of 
      constitutive MCP-1 from AH patient monocytes was 4694+/-2432 pg/ml 20 h on 
      admission. The mean MCP-1 level for LPS-treated monocytes was 4903+/-1540 pg/ml 
      20 h for normal subjects and was significantly elevated in AH patients to 
      11589+/-3266 pg/ml/20 h. AH patient monocyte MCP-1 production was decreased in 
      vitro when monocytes were treated with N-acetylcysteine (5 mM) and also decreased 
      over the 6-month study as the patients improved clinically. MCP-1 plasma levels 
      were below the detection limits of the assay used in both AH patients and normal 
      subjects. Thus, monocytes from AH patients not only constitutively product MCP-1, 
      but also produce higher levels of MCP-1 with endotoxin stimulation. Further 
      studies are needed to clarify the role of MCP-1 in the activation and hepatic 
      infiltration of mononuclear cells in alcoholic liver disease.
CI  - Copyright 1999 Academic Press.
FAU - Devalaraja, M N
AU  - Devalaraja MN
AD  - Graduate Center for Toxicology, University of Kentucky.
FAU - Mcclain, C J
AU  - Mcclain CJ
FAU - Barve, S
AU  - Barve S
FAU - Vaddi, K
AU  - Vaddi K
FAU - Hill, D B
AU  - Hill DB
LA  - eng
GR  - 1K20AA00190-01/AA/NIAAA NIH HHS/United States
GR  - 1K21 AA0205-01/AA/NIAAA NIH HHS/United States
GR  - 1PO1 NS31220-01A1/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Lipopolysaccharides)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Acute Disease
MH  - Adult
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Culture Media, Conditioned/metabolism
MH  - Female
MH  - Hepatitis, Alcoholic/*metabolism
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation/drug effects
MH  - Male
MH  - Monocytes/drug effects/*metabolism
MH  - Time Factors
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S1043-4666(99)90495-7 [pii]
AID - 10.1006/cyto.1999.0495 [doi]
PST - ppublish
SO  - Cytokine. 1999 Nov;11(11):875-81. doi: 10.1006/cyto.1999.0495.