PMID- 10547578
OWN - NLM
STAT- MEDLINE
DCOM- 20000309
LR  - 20151119
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 189
IP  - 2
DP  - 1999 Oct
TI  - Androgen receptor gene amplification increases tissue PSA protein expression in 
      hormone-refractory prostate carcinoma.
PG  - 219-23
AB  - Androgen receptor (AR) gene amplification was analysed by fluorescence in situ 
      hybridization (FISH) from 24 paraffin-embedded prostate carcinoma samples 
      recurring locally during hormonal therapy and prostate-specific antigen (PSA) 
      expression from 15/24 of these samples was studied by immunohistochemistry (IHC). 
      AR gene amplification was detected in 29 per cent (7/24) of the recurrent 
      tumours. Using modified Histoscore (MHS), PSA immunostaining in the AR 
      gene-amplified tumours (133+/-102) was twice as high (p=0.054) as in tumours with 
      no amplification (66+/-79) and a statistically significant (p=0.026) association 
      between AR gene amplification and PSA positivity was found when MHS>/=20 was 
      considered positive for PSA. AR gene copy number was positively correlated with 
      PSA MHS in the AR gene-amplified tumours (r=0.893, p=0.012). Histological grade, 
      Gleason's score, and tumour stage did not differ significantly between patients 
      with and without AR gene amplification. In conclusion, these results indicate 
      that AR gene amplification leads to up-regulation of PSA gene (and possibly other 
      androgen-dependent genes), and that patients with AR gene amplification may have 
      elevated serum PSA concentrations without a clear correlation with actual tumour 
      burden.
CI  - Copyright 1999 John Wiley & Sons, Ltd.
FAU - Koivisto, P A
AU  - Koivisto PA
AD  - Laboratory of Cancer Genetics, Department of Clinical Chemistry, Tampere 
      University Hospital, P.O. Box 2000, FIN-33521 Tampere, Finland. blpako@uta.fi
FAU - Helin, H J
AU  - Helin HJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Receptors, Androgen)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
MH  - Biomarkers, Tumor/*metabolism
MH  - Gene Amplification
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasm Recurrence, Local/*genetics/metabolism/therapy
MH  - Prostate-Specific Antigen/*metabolism
MH  - Prostatic Neoplasms/*genetics/metabolism/therapy
MH  - Receptors, Androgen/*genetics
MH  - Treatment Failure
EDAT- 1999/11/05 08:00
MHDA- 2000/03/11 09:00
CRDT- 1999/11/05 08:00
PHST- 1999/11/05 08:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 1999/11/05 08:00 [entrez]
AID - 10.1002/(SICI)1096-9896(199910)189:2<219::AID-PATH423>3.0.CO;2-F [pii]
AID - 10.1002/(SICI)1096-9896(199910)189:2<219::AID-PATH423>3.0.CO;2-F [doi]
PST - ppublish
SO  - J Pathol. 1999 Oct;189(2):219-23. doi: 
      10.1002/(SICI)1096-9896(199910)189:2<219::AID-PATH423>3.0.CO;2-F.