PMID- 10548727
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20220129
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 239
IP  - 2
DP  - 1999 Nov 1
TI  - Analysis of NotI linking clones isolated from human chromosome 3 specific 
      libraries.
PG  - 259-71
AB  - We have partially sequenced more than 1000 NotI linking clones isolated from 
      human chromosome 3-specific libraries. Of these clones, 152 were unique 
      chromosome 3-specific clones. The clones were precisely mapped using a 
      combination of fluorescence in situ hybridization (FISH) and hybridization to 
      somatic cell or radiation hybrids. Two- and three-color FISH was used to order 
      the clones that mapped to the same chromosomal region, and in some cases, 
      chromosome jumping was used to resolve ambiguous mapping. When this NotI 
      restriction map was compared with the yeast artificial chromosome (YAC) based 
      chromosome 3 map, significant differences in several chromosome 3 regions were 
      observed. A search of the EMBL nucleotide database with these sequences revealed 
      homologies (90-100%) to more than 100 different genes or expressed sequence tags 
      (ESTs). Many of these homologies were used to map new genes to chromosome 3. 
      These results suggest that sequencing NotI linking clones, and sequencing CpG 
      islands in general, may complement the EST project and aid in the discovery of 
      all human genes by sequencing random cDNAs. This method may also yield 
      information that cannot be obtained by the EST project alone; namely, the 
      identification of the 5' ends of genes, including potential promoter/enhancer 
      regions and other regulatory sequences
FAU - Kashuba, V I
AU  - Kashuba VI
AD  - Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden. 
      vlakas@ki.se
FAU - Gizatullin, R Z
AU  - Gizatullin RZ
FAU - Protopopov, A I
AU  - Protopopov AI
FAU - Li, J
AU  - Li J
FAU - Vorobieva, N V
AU  - Vorobieva NV
FAU - Fedorova, L
AU  - Fedorova L
FAU - Zabarovska, V I
AU  - Zabarovska VI
FAU - Muravenko, O V
AU  - Muravenko OV
FAU - Kost-Alimova, M
AU  - Kost-Alimova M
FAU - Domninsky, D A
AU  - Domninsky DA
FAU - Kiss, C
AU  - Kiss C
FAU - Allikmets, R
AU  - Allikmets R
FAU - Zakharyev, V M
AU  - Zakharyev VM
FAU - Braga, E A
AU  - Braga EA
FAU - Sumegi, J
AU  - Sumegi J
FAU - Lerman, M
AU  - Lerman M
FAU - Wahlestedt, C
AU  - Wahlestedt C
FAU - Zelenin, A V
AU  - Zelenin AV
FAU - Sheer, D
AU  - Sheer D
FAU - Winberg, G
AU  - Winberg G
FAU - Grafodatsky, A
AU  - Grafodatsky A
FAU - Kisselev, L L
AU  - Kisselev LL
FAU - Klein, G
AU  - Klein G
FAU - Zabarovsky, E R
AU  - Zabarovsky ER
LA  - eng
GR  - A3585/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GCGGCCGC-specific type II deoxyribonucleases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - DNA/chemistry/*genetics/metabolism
MH  - Databases, Factual
MH  - Deoxyribonucleases, Type II Site-Specific/*metabolism
MH  - Expressed Sequence Tags
MH  - *Gene Library
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S0378111999004114 [pii]
AID - 10.1016/s0378-1119(99)00411-4 [doi]
PST - ppublish
SO  - Gene. 1999 Nov 1;239(2):259-71. doi: 10.1016/s0378-1119(99)00411-4.