PMID- 10549264
OWN - NLM
STAT- MEDLINE
DCOM- 19991117
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 114
IP  - 2
DP  - 1999 Oct 15
TI  - Clinical implications of fluorescence in situ hybridization analysis in 13 
      chronic myeloid leukemia cases: Ph-negative and variant Ph-positive.
PG  - 100-7
AB  - Thirteen chronic myeloid leukemia (CML) patients, 10 with variant Philadelphia 
      (Ph) translocations and 3 Ph negative cases, were analyzed by fluorescence in 
      situ hybridization (FISH) with the use of BCR and ABL cosmid probes and a 
      chromosome 22 painting probe. In the variant Ph translocations, the BCR-ABL 
      fusion gene was located on the Ph chromosome; in 1 CML Ph-negative patient, the 
      BCR-ABL fusion gene was located on the Ph chromosome; and, in 2 patients, it was 
      located on chromosome 9. The chromosome 22 painting probe was detected on the 
      third-party chromosome of the variant translocation, and in none of the variant 
      translocations was there any detectable signal on chromosome 9. In CML patients 
      with clonal evolution of a simple Ph, a signal of the chromosome 22 painting 
      probe was detected on the der(9) of the Ph translocation. It was concluded that 
      the variant Ph translocations evolved simultaneously in a three-way 
      rearrangement. The clinical parameters of the 13 patients were similar to those 
      of a large group of CML patients with a simple Ph translocation. It is suggested 
      that, to determine the prognosis of CML patients with a complex karyotype, FISH 
      analysis with a chromosome 22 painting probe be performed.
FAU - Yehuda, O
AU  - Yehuda O
AD  - Department of Human Genetics, Hadassah Hebrew University Hospital, Hadassah 
      Hebrew University Medical School, Jerusalem, Israel.
FAU - Abeliovich, D
AU  - Abeliovich D
FAU - Ben-Neriah, S
AU  - Ben-Neriah S
FAU - Sverdlin, I
AU  - Sverdlin I
FAU - Cohen, R
AU  - Cohen R
FAU - Varadi, G
AU  - Varadi G
FAU - Orr, R
AU  - Orr R
FAU - Ashkenazi, Y J
AU  - Ashkenazi YJ
FAU - Heyd, J
AU  - Heyd J
FAU - Lugassy, G
AU  - Lugassy G
FAU - Ben Yehuda, D
AU  - Ben Yehuda D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Painting
MH  - Chromosomes, Human, Pair 22/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Genetic Variation/*genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/mortality/pathology
MH  - Leukemia, Myeloid, Chronic, Atypical, BCR-ABL 
      Negative/*genetics/mortality/pathology
MH  - Male
MH  - Middle Aged
MH  - *Philadelphia Chromosome
MH  - Prognosis
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S0165460899000679 [pii]
AID - 10.1016/s0165-4608(99)00067-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Oct 15;114(2):100-7. doi: 
      10.1016/s0165-4608(99)00067-9.