PMID- 10549271
OWN - NLM
STAT- MEDLINE
DCOM- 19991117
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 114
IP  - 2
DP  - 1999 Oct 15
TI  - Amplification of C-MYC as the origin of the homogeneous staining region in 
      ovarian carcinoma detected by micro-FISH.
PG  - 136-43
AB  - Homogeneous staining region (hsr), a cytogenetic indicator of gene amplification, 
      has been frequently found in ovarian carcinoma (ovc). To identify the origin of 
      the hsr, chromosome microdissection combined with polymerase chain reaction and 
      fluorescence in situ hybridization (FISH) was applied to two human ovarian cancer 
      cell lines, GR and MLS/P. The hsr probes were labeled with biotin or digoxigenin 
      and hybridized to normal metaphase spreads to elucidate the chromosomal origin 
      and regional localization of the amplified genes. FISH to normal metaphase 
      spreads with the probe generated from the whole hsr-bearing chromosome from GR 
      hybridized to 8q24, 2p13-->2q11.2, 10pter-->10p15, 10p12-->10q11.2, 5q23-->5q31, 
      and 5q33-->5qter. For MLS/P, the hsr-bearing marker chromosome hybridized to 8q 
      and 15q. In both cases, detailed FISH analysis revealed enhanced signal intensity 
      at the 8q24 locus, which coincides with the chromosomal location of the C-MYC 
      oncogene. To verify the involvement of C-MYC in hsr formation, in situ 
      hybridization with a probe specific for the C-MYC oncogene was conducted and 
      confirmed the amplification of C-MYC as the origin of the hsr. The whole 
      hsr-bearing chromosome for GR is designated as rev ish der(10) 
      (10pter-->10p15::8q24hsr:: 10p12-->10q11.2::8q24::2q11.2-->2p13::2p13 
      -->2q11.2::8q24::10q11-->10p11.2:: 5q23-->5q31::5q33-->5qter 
      (wcp10+,D10Z1++,wcp2+,D2Z++,wcp5+,wcp8+ ,C-MYC++/hsr). The hsr-bearing marker for 
      MLS/P is designated as rev ish der(8)(qter-->8q24::8q24::8q24-->8q10:: 
      8q10-->8q24::8q24::8q24-->8qter:: 15q11-->15qter)(wcp8+, D8Z1+,wcp15+,C-MYC++. 
      FISH with the probe generated from the hsr of GR also painted the hsr in MLS/P, 
      indicating that the two hsrs have shared homology, which indicates that the 
      amplification of 8q24/C-MYC as the origin of hsr may be a nonrandom genomic 
      alteration in ovc.
FAU - Abeysinghe, H R
AU  - Abeysinghe HR
AD  - Department of Pathology, University of Rochester School of Medicine, New York 
      14642, USA.
FAU - Cedrone, E
AU  - Cedrone E
FAU - Tyan, T
AU  - Tyan T
FAU - Xu, J
AU  - Xu J
FAU - Wang, N
AU  - Wang N
LA  - eng
GR  - CA 52761/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Chromosome Banding
MH  - Chromosomes, Human/genetics
MH  - DNA Probes
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Genes, myc/*genetics
MH  - Genetic Markers/genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Ovarian Neoplasms/*genetics
MH  - Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S0165460899000643 [pii]
AID - 10.1016/s0165-4608(99)00064-3 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Oct 15;114(2):136-43. doi: 
      10.1016/s0165-4608(99)00064-3.