PMID- 10549274
OWN - NLM
STAT- MEDLINE
DCOM- 19991117
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 114
IP  - 2
DP  - 1999 Oct 15
TI  - Evidence for in vitro selection during cell culturing of breast cancer: detection 
      by flow and image cytometry.
PG  - 154-5
AB  - Detailed studies of chromosome rearrangements within solid tumors require 
      karyotype analysis after cell culturing. However, different cell subpopulations 
      with various growth capacities within one tumor may introduce biases in karyotype 
      analysis, known as the in vitro selection. In our laboratory, 22% of karyotypes 
      from breast cancers established after short-term culture were normal. Using 
      interphase fluorescence in situ hybridization (FISH) for the determination of 
      chromosome 1 arm imbalances and flow cytometry measurements of ploidy, we 
      demonstrated that at least 2/3 of these tumors were mainly composed of aneuploid 
      cell populations. Thus, the incidence of normal or balanced karyotypes among 
      breast cancers is probably below 7%. This is the first direct proof for the 
      existence of an in vitro selection within breast cancer cultures, suggesting 
      cautious interpretation of cytogenetic data.
FAU - Truong, K
AU  - Truong K
AD  - Institut Curie, Paris, France.
FAU - Guilly, M N
AU  - Guilly MN
FAU - Gerbault-Seureau, M
AU  - Gerbault-Seureau M
FAU - Malfoy, B
AU  - Malfoy B
FAU - Vielh, P
AU  - Vielh P
FAU - Dutrillaux, B
AU  - Dutrillaux B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aneuploidy
MH  - Artifacts
MH  - Breast Neoplasms/*genetics/*pathology
MH  - Carcinoma, Ductal, Breast/genetics/pathology
MH  - Carcinoma, Intraductal, Noninfiltrating/genetics/pathology
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Female
MH  - *Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Middle Aged
MH  - Ploidies
MH  - Selection, Genetic
MH  - Time Factors
MH  - Tumor Cells, Cultured
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
AID - S0165460899000527 [pii]
AID - 10.1016/s0165-4608(99)00052-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Oct 15;114(2):154-5. doi: 
      10.1016/s0165-4608(99)00052-7.