PMID- 10549600
OWN - NLM
STAT- MEDLINE
DCOM- 19991115
LR  - 20190910
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 75
IP  - 10
DP  - 1999 Oct
TI  - Stable chromosomal aberrations in haemopoietic stem cells in the blood of 
      radiation accident victims.
PG  - 1241-50
AB  - PURPOSE: The detection of long-term persistent chromosome aberrations in 
      circulating haemopoietic stem cells after accidental radiation exposure. MATERIAL 
      AND METHODS: Peripheral blood samples from highly exposed persons were collected 
      7-25 years after the radiation accidents in Moscow (1971), Kazan (1975) and 
      Chernobyl (1996). Haemopoietic blood stem cells were analysed when investigating 
      individual colonies derived from haemopoietic progenitor cells: burst-forming 
      units-erythroid (BFU-E), granulocyte-macrophage-colony-forming cells (GM-CFC) and 
      multipotent granulocyte-erythrocyte-macrophage- megakaryocyte-colony-forming 
      cells (GEMM-CFC). Colony formation was obtained in methylcellulose cultures. 
      Chromosome preparations in single colonies were performed using a microtechnique. 
      RESULTS: Nine patients were investigated at 1 to 4 follow-up time points after 
      radiation exposure. Three hundred and thirty-four single colonies were analyzed 
      resulting in 1375 mitoses. It was found that colonies showed chromosome 
      aberrations (ChA) up to 25 years after radiation exposure by classical 
      cytogenetics and by fluorescence in situ hybridization (FISH). Stable aberrations 
      were detected in 21% of colonies. They were clonal in 19% of colonies, i.e. the 
      same abnormality was found in all cells derived from a single colony. In 2% of 
      colonies ChA were stable but non-clonal; unstable ChA were not observed. 
      CONCLUSIONS: The results indicate that blood-derived haemopoietic stem cells may 
      serve as a biological indicator to detect radiation-induced ChA. Since they are 
      considered to be in dynamic and functional exchange with stem cells in the 
      medullary sites of blood cell formation such as bone marrow, the use of blood 
      stem cells as a marker of radiation effects should be explored to assess the 
      repair status of the stem cell pool as such.
FAU - Kreja, L
AU  - Kreja L
AD  - Institute for Occupational- Social- and Environmental Medicine, University of 
      Ulm, Germany. ludwika.kreja@medizin.uni-ulm.de
FAU - Greulich, K M
AU  - Greulich KM
FAU - Fliedner, T M
AU  - Fliedner TM
FAU - Heinze, B
AU  - Heinze B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Colony-Forming Units Assay
MH  - Cytogenetics
MH  - Female
MH  - Hematopoietic Stem Cells/*radiation effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Moscow
MH  - Occupational Exposure
MH  - *Radioactive Hazard Release
MH  - Russia
MH  - Time Factors
MH  - Ukraine
EDAT- 1999/11/30 00:00
MHDA- 1999/11/30 00:01
CRDT- 1999/11/30 00:00
PHST- 1999/11/30 00:00 [pubmed]
PHST- 1999/11/30 00:01 [medline]
PHST- 1999/11/30 00:00 [entrez]
AID - 10.1080/095530099139395 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1999 Oct;75(10):1241-50. doi: 10.1080/095530099139395.