PMID- 10549663
OWN - NLM
STAT- MEDLINE
DCOM- 19991108
LR  - 20220309
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 40
IP  - 12
DP  - 1999 Nov
TI  - Patterned expression of BDNF and NT-3 in the retina and anterior segment of the 
      developing mammalian eye.
PG  - 2996-3005
AB  - PURPOSE: The neurotrophins brain-derived neurotrophic factor (BDNF) and 
      neurotrophin-3 (NT-3) are hypothesized to play an important role in vertebrate 
      eye development because of their patterned expression in the developing and adult 
      neuroretina, their regulated response to retinal and optic nerve injury, and the 
      effects of altered neurotrophin signaling on retinal development. To further 
      characterize the role of these neurotrophins in mammalian eye development and 
      maintenance, the pattern of expression of BDNF and NT-3 was analyzed in the 
      developing and mature mouse eye. METHODS: Using mouse strains in which the 
      reporter gene lacZ, encoding the enzyme beta-galactosidase, was targeted to 
      either the BDNF or NT-3 locus, the expression of BDNF and NT-3 in the eyes of 
      mice heterozygous for these mutations was analyzed by enzyme histochemistry 
      during embryogenesis, postnatal development, and adulthood. RESULTS: BDNF and 
      NT-3 expression were first observed in the inner and outer segments of the 
      developing optic cup at embryonic days 10.5 to 11.5. As the retina matured, BDNF 
      expression was restricted to retinal ganglion cells and a subset of cells in the 
      inner nuclear layer (INL), whereas NT-3 expression was confined to a small subset 
      of cells in the INL and ganglion cell layer. Both neurotrophins were expressed 
      within the developing retinal pigment epithelium. In the anterior segment, BDNF 
      and NT-3 were expressed at high levels in the developing and mature ciliary 
      epithelium. In the lens and cornea, however, these neurotrophins displayed 
      distinct patterns of expression during development and adulthood. BDNF expression 
      was found in the lens epithelium, immature trabecular meshwork, corneal 
      endothelium, and corneal epithelium, whereas NT-3 expression was confined to the 
      corneal epithelium. CONCLUSIONS: BDNF and NT-3 exhibit different, yet 
      overlapping, patterns of expression during the development and differentiation of 
      the mouse eye. In addition to the neuroretina, the spatiotemporal expression of 
      BDNF and NT-3 may play an important role in the development and maintenance of 
      the lens, ciliary body, trabecular meshwork, and cornea.
FAU - Bennett, J L
AU  - Bennett JL
AD  - Department of Neurology, University of Colorado Health Sciences Center, Denver 
      80262, USA. jeffrey.bennett@uchsc.edu
FAU - Zeiler, S R
AU  - Zeiler SR
FAU - Jones, K R
AU  - Jones KR
LA  - eng
GR  - K08EY00387-02/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Anterior Eye Segment/*embryology/growth & development/metabolism
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Cell Differentiation
MH  - Embryonic and Fetal Development
MH  - Eye/growth & development
MH  - Histocytochemistry
MH  - Lac Operon
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Neurotrophin 3/*biosynthesis
MH  - Retina/*embryology/growth & development/metabolism
MH  - beta-Galactosidase/metabolism
EDAT- 1999/11/05 00:00
MHDA- 1999/11/05 00:01
CRDT- 1999/11/05 00:00
PHST- 1999/11/05 00:00 [pubmed]
PHST- 1999/11/05 00:01 [medline]
PHST- 1999/11/05 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1999 Nov;40(12):2996-3005.