PMID- 10555160
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20190915
IS  - 0168-0102 (Print)
IS  - 0168-0102 (Linking)
VI  - 35
IP  - 1
DP  - 1999 Oct
TI  - Brain-derived neurotrophic factor, nerve growth and neurotrophin-3 selected 
      regions of the rat brain following kainic acid-induced seizure activity.
PG  - 19-29
AB  - Changes in levels of brain-derived neurotrophic factor (BDNF), nerve growth 
      factor (NGF) and neurotrophin-3 (NT-3) in various regions of the rat brain 
      following kainic acid-induced seizure activity were investigated. BDNF protein, 
      as measured by a two-site enzyme immunoassay, increased transiently 12-24 h after 
      the intraperitoneal administration of kainic acid to 61.6 ng/g wet weight in the 
      hippocampus (approximately 10-fold increase), 19.5 ng/g in the piriform plus 
      entorhinal cortex (approximately 10-fold) and 8.2 ng/g in the olfactory bulb 
      (approximately 16-fold), and then rapidly decreased. Increases of 2- to 4-fold in 
      levels of BDNF were also detected in the septum, cerebral cortex, striatum and 
      hypothalamus, but not in the cerebellum. In contrast, levels of NGF and NT-3 
      decreased 24 h after the administration of kainic acid. Western and Northern 
      blotting analyses of hippocampal tissues, respectively, revealed increase in 
      levels of a 14-kDa protein corresponding to BDNF and its mRNA at both 4.2 and 1.4 
      kb. Hippocampal mRNAs for NGF and NT-3 increased and decreased, respectively, in 
      kainic acid-treated rats. Immunohistological investigations showed that, in the 
      hippocampus, the administration of kainic acid enhanced a homogeneous 
      immunoreactivity of BDNF in the polymorph inner layer (the stratum radiatum of 
      the CA3/CA4 regions and the hilar region) and in granule cells of the dentate 
      gyrus. BDNF protein was found in neurons, but not at all in glial cells or in 
      blood vessels, and was localized in the cytoplasm, the nucleoplasm and the 
      primary dendrites of neurons as well as in perisynaptic extracellular spaces, but 
      hardly in their axons. Our results show that kainic acid treatment increases 
      levels of BDNF, but not NGF or NT-3, in various regions of the rat brain, other 
      than the cerebellum. Also, the majority of BDNF newly synthesized by hippocampal 
      granule neurons is secreted into the perisynaptic extracellular space in the 
      polymorph inner layer of the dentate gyrus, supporting an autocrine-like role for 
      the factor in synaptic functions.
FAU - Katoh-Semba, R
AU  - Katoh-Semba R
AD  - Department of Perinatology, Institute for Developmental Research, Aichi Human 
      Service Center, Kasugai, Japan. katohsemba@inst-hsc.pref.aichi.jp
FAU - Takeuchi, I K
AU  - Takeuchi IK
FAU - Inaguma, Y
AU  - Inaguma Y
FAU - Ito, H
AU  - Ito H
FAU - Kato, K
AU  - Kato K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Brain/drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Female
MH  - *Gene Expression Regulation/drug effects
MH  - Immunohistochemistry
MH  - Kainic Acid/*toxicity
MH  - Kinetics
MH  - Male
MH  - Nerve Growth Factors/*genetics/metabolism
MH  - Neurotrophin 3/*genetics/metabolism
MH  - Organ Specificity
MH  - Pituitary Gland/drug effects/metabolism
MH  - RNA, Messenger/analysis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seizures/chemically induced/*metabolism
MH  - Time Factors
MH  - Transcription, Genetic
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - S0168010299000590 [pii]
AID - 10.1016/s0168-0102(99)00059-0 [doi]
PST - ppublish
SO  - Neurosci Res. 1999 Oct;35(1):19-29. doi: 10.1016/s0168-0102(99)00059-0.