PMID- 10557043
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 13
IP  - 11
DP  - 1999 Nov
TI  - Comparison of cytogenetics, Southern blotting, and fluorescence in situ 
      hybridization as methods for detecting MLL gene rearrangements in children with 
      acute leukemia and with 11q23 abnormalities.
PG  - 1713-20
AB  - Bone marrow samples from 67 children with acute leukemia and with cytogenetic 
      evidence of chromosome 11 band q23 (11q23) abnormalities were characterized by 
      fluorescence in situ hybridization (FISH) and Southern blot analysis to determine 
      whether FISH could reliably detect MLL gene rearrangements in this population. 
      Among the 42 patients with acute lymphoblastic leukemia (ALL), MLL gene 
      rearrangements were detected in cells from 23 patients (54.8%) by both FISH and 
      Southern blot analysis. FISH identified allelic deletions of MLL gene in five of 
      12 patients (42%) with ALL and with deletion of 11q23. In 22 of 25 children (88%) 
      with AML, FISH detected MLL gene rearrangements, whereas Southern blot analysis 
      identified rearrangements in 24 of 25 patients (96%). For children with acute 
      leukemia and with 11q23 abnormalities, we recommend that FISH be used for the 
      rapid screening of MLL gene rearrangements and that Southern blot analysis be 
      used for the definitive assessment of the MLL gene status.
FAU - Mathew, S
AU  - Mathew S
AD  - Department of Pathology and Laboratory Medicine, St Jude Children's Research 
      Hospital, 332 North Lauderdale Street, Memphis, TN 38105-2794, USA.
FAU - Behm, F
AU  - Behm F
FAU - Dalton, J
AU  - Dalton J
FAU - Raimondi, S
AU  - Raimondi S
LA  - eng
GR  - CA-20180/CA/NCI NIH HHS/United States
GR  - CA-21765/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Alleles
MH  - *Blotting, Southern
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Breakage/genetics
MH  - Chromosome Deletion
MH  - Chromosome Inversion
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Infant, Newborn
MH  - Leukemia, Myeloid/diagnosis/genetics
MH  - Male
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/*genetics
MH  - *Proto-Oncogenes
MH  - *Transcription Factors
MH  - Translocation, Genetic/genetics
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - 10.1038/sj.leu.2401512 [doi]
PST - ppublish
SO  - Leukemia. 1999 Nov;13(11):1713-20. doi: 10.1038/sj.leu.2401512.