PMID- 10558876
OWN - NLM
STAT- MEDLINE
DCOM- 19991220
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 265
IP  - 2
DP  - 1999 Nov 19
TI  - Hypoxia regulates the expression of the adrenomedullin and HIF-1 genes in 
      cultured HL-1 cardiomyocytes.
PG  - 382-6
AB  - Adrenomedullin (AM) is a hypotensive protein expressed in a variety of cells and 
      tissues. We observed previously that the expression of the adrenomedullin gene 
      increases substantially in the developing rat heart and in cultured adult rat 
      ventricular cardiac myocytes in response to hypoxia as a function of time. An 
      adrenomedullin promoter-luciferase reporter construct was used to show that this 
      increase in adrenomedullin mRNA resulted from increased transcription in response 
      to hypoxia. We report here additional evidence documenting that this 
      hypoxia-induced transcription of the adrenomedullin gene is regulated by the 
      hypoxia-inducible factor-1 (HIF-1) transcription factor. We used Northern blot 
      analysis to show an increase in the levels of AM and HIF-1alpha mRNA but not 
      HIF-1beta mRNA in the HL-1 cardiac myocyte cell line in response to hypoxia. 
      Furthermore, Western blot analysis revealed that the levels of both HIF-1alpha 
      and HIF-1beta protein increased under hypoxic conditions. Data from 
      electrophoretic mobility shift assays indicate that the heterodimeric HIF-1 
      complex binds to the HIF-1-responsive elements. Combined data from these studies 
      demonstrate that the AM gene is regulated by hypoxia-responsive elements 
      localized in the AM promoter region.
CI  - Copyright 1999 Academic Press.
FAU - Nguyen, S V
AU  - Nguyen SV
AD  - Department of Biochemistry and Molecular Biology, Louisiana State University 
      Health Science Center, New Orleans, Louisiana, 70112, USA.
FAU - Claycomb, W C
AU  - Claycomb WC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Hif1a protein, mouse)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Lymphokines)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 148498-78-6 (Adrenomedullin)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Adrenomedullin
MH  - Animals
MH  - Base Sequence
MH  - Cell Hypoxia/*genetics
MH  - Cell Line
MH  - DNA Primers/genetics
MH  - DNA-Binding Proteins/chemistry/*genetics/metabolism
MH  - Endothelial Growth Factors/genetics
MH  - Gene Expression Regulation
MH  - Genes, Reporter
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Luciferases/genetics
MH  - Lymphokines/genetics
MH  - Mice
MH  - Myocardium/cytology/*metabolism
MH  - Nuclear Proteins/chemistry/*genetics/metabolism
MH  - Peptides/*genetics
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - *Transcription Factors
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S0006-291X(99)91674-1 [pii]
AID - 10.1006/bbrc.1999.1674 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Nov 19;265(2):382-6. doi: 
      10.1006/bbrc.1999.1674.