PMID- 10559401
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20220310
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 22
DP  - 1999 Nov 15
TI  - Brain-derived neurotrophic factor differentially regulates retinal ganglion cell 
      dendritic and axonal arborization in vivo.
PG  - 9928-38
AB  - Expression of the neurotrophin brain-derived neurotrophic factor (BDNF) and its 
      receptor trkB in the ganglion cell layer of the Xenopus retina during retinal 
      ganglion cell (RGC) dendritic arborization indicates that BDNF is spatially and 
      temporally available to influence RGC morphological differentiation (; ). BDNF 
      promotes RGC axon arborization in vivo by acting as a target-derived trophic 
      factor (). To determine whether BDNF also acts locally to regulate RGC dendritic 
      development in vivo, we altered retinal neurotrophin levels at the onset of 
      dendritic arborization and assessed the resulting arbor morphologies of RGCs 
      retrogradely labeled with fluorescent dextrans. Injecting neurotrophins or BDNF 
      function-blocking antibodies coupled to microspheres provided local alterations 
      of retinal neurotrophin levels. BDNF significantly decreased RGC dendritic arbor 
      complexity, whereas neutralizing endogenous BDNF levels with function-blocking 
      antibodies significantly increased dendritic arbor complexity. RGCs exposed to 
      other neurotrophins, as well as RGCs in retinae treated with BDNF but in areas 
      not directly exposed to the neurotrophin, developed dendritic arbors that were 
      indistinguishable from controls, indicating that exogenous BDNF acts specifically 
      and locally. In the tectum, where RGC axons arborize, BDNF had opposite effects. 
      BDNF significantly increased RGC axon arbor complexity and anti-BDNF reduced RGC 
      arborization. Thus, BDNF reduces RGC dendritic arborization within the retina and 
      increases axon arborization in the tectum. These results indicate that BDNF can 
      differentially modulate axonal and dendritic arborization within a single 
      neuronal population in opposing manners and raise the possibility that 
      differential modulation by a neurotrophic factor finely tunes the morphological 
      differentiation program of a neuron.
FAU - Lom, B
AU  - Lom B
AD  - Mental Retardation Research Center, Departments of Psychiatry and Neurobiology, 
      University of California, Los Angeles, Los Angeles, California 90025, USA.
FAU - Cohen-Cory, S
AU  - Cohen-Cory S
LA  - eng
GR  - R01 EY011912/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Axons/drug effects/physiology/*ultrastructure
MH  - Brain-Derived Neurotrophic Factor/immunology/*pharmacology/physiology
MH  - Cell Differentiation
MH  - Dendrites/drug effects/physiology/*ultrastructure
MH  - Embryo, Nonmammalian/physiology
MH  - Female
MH  - Nerve Growth Factors/*pharmacology
MH  - Neurotrophin 3/pharmacology
MH  - Retinal Ganglion Cells/drug effects/physiology/*ultrastructure
MH  - Xenopus laevis/embryology
PMC - PMC6782992
EDAT- 1999/11/13 00:00
MHDA- 1999/11/13 00:01
PMCR- 2000/05/15
CRDT- 1999/11/13 00:00
PHST- 1999/11/13 00:00 [pubmed]
PHST- 1999/11/13 00:01 [medline]
PHST- 1999/11/13 00:00 [entrez]
PHST- 2000/05/15 00:00 [pmc-release]
AID - 3625 [pii]
AID - 10.1523/JNEUROSCI.19-22-09928.1999 [doi]
PST - ppublish
SO  - J Neurosci. 1999 Nov 15;19(22):9928-38. doi: 10.1523/JNEUROSCI.19-22-09928.1999.