PMID- 10559407
OWN - NLM
STAT- MEDLINE
DCOM- 19991202
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 22
DP  - 1999 Nov 15
TI  - BDNF modulates, but does not mediate, activity-dependent branching and remodeling 
      of optic axon arbors in vivo.
PG  - 9996-10003
AB  - The proper development of axon terminal arbors and their recognition of target 
      neurons depend, in part, on neuronal activity. Neurotrophins are attractive 
      candidate signals to participate in activity-dependent development and refinement 
      of neuronal connectivity. In the visual system, brain-derived neurotrophic factor 
      (BDNF) has been shown to modulate the elaboration and refinement of axonal arbors 
      and to participate in the establishment of topographically ordered visual maps. 
      By examining in vivo with time-lapse microscopy the effects of activity blockade 
      and BDNF on optic axon arborization, I show that the dynamic mechanisms by which 
      neurotrophins and neuronal activity regulate axon arborization differ. Acute 
      retinal activity blockade by intraocular injection of tetrodotoxin (TTX) rapidly 
      and significantly increased branch addition and elimination, thus interfering 
      with axon branch stabilization. The effects of activity blockade on branch 
      dynamics resulted in increased arbor complexity in the long term and were 
      prevented by altering endogenous BDNF levels at the target. BDNF promoted axon 
      arborization by increasing branch addition and lengthening, without affecting 
      branch elimination. Activity blockade, however, did not prevent the 
      growth-promoting effects of BDNF, indicating that BDNF can affect axon 
      arborization even in the absence of activity. Together this evidence indicates 
      that BDNF acts as a modulator, but not as a direct mediator, of activity during 
      the morphological development of neurons. Consequently, neuronal activity and 
      BDNF use distinct but interactive mechanisms to control the development of 
      neuronal connectivity; BDNF modulates axon arborization by promoting growth, 
      neuronal activity participates in axon branch stabilization, and together these 
      two signals converge to shape axon form.
FAU - Cohen-Cory, S
AU  - Cohen-Cory S
AD  - Mental Retardation Research Center, Departments of Psychiatry and Neurobiology, 
      University of California, Los Angeles, Los Angeles, California 90095, USA. 
      scohenco@ucla.edu
LA  - eng
GR  - R01 EY011912/EY/NEI NIH HHS/United States
GR  - EY11912/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Recombinant Proteins)
RN  - 4368-28-9 (Tetrodotoxin)
SB  - IM
MH  - Action Potentials/drug effects/physiology
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Axons/drug effects/physiology/*ultrastructure
MH  - Brain-Derived Neurotrophic Factor/*pharmacology/*physiology
MH  - Humans
MH  - Photic Stimulation
MH  - Recombinant Proteins/pharmacology
MH  - Retinal Ganglion Cells/drug effects/physiology/*ultrastructure
MH  - Tetrodotoxin/pharmacology
MH  - Xenopus laevis
PMC - PMC6782987
EDAT- 1999/11/13 00:00
MHDA- 1999/11/13 00:01
PMCR- 2000/05/15
CRDT- 1999/11/13 00:00
PHST- 1999/11/13 00:00 [pubmed]
PHST- 1999/11/13 00:01 [medline]
PHST- 1999/11/13 00:00 [entrez]
PHST- 2000/05/15 00:00 [pmc-release]
AID - 3603 [pii]
AID - 10.1523/JNEUROSCI.19-22-09996.1999 [doi]
PST - ppublish
SO  - J Neurosci. 1999 Nov 15;19(22):9996-10003. doi: 
      10.1523/JNEUROSCI.19-22-09996.1999.