PMID- 10562464
OWN - NLM
STAT- MEDLINE
DCOM- 20000107
LR  - 20220318
IS  - 1096-7192 (Print)
IS  - 1096-7192 (Linking)
VI  - 68
IP  - 3
DP  - 1999 Nov
TI  - Expression of hexokinase 1 and hexokinase 2 in mammary tissue of nonlactating and 
      lactating rats: evaluation by RT-PCR.
PG  - 371-4
AB  - Because the initial step in the metabolism of glucose involves phosphorylation by 
      hexokinase (HK), we tested the hypothesis that the expression of the isozymes, 
      hexokinase type 1 (HK1) and hexokinase type 2 (HK2), would be different in rat 
      mammary tissue during pregnancy and lactation. RNA was extracted from mammary 
      tissue dissected from timed pregnant rats (from gestional days 10 to 21) and 
      nursing rat mothers (up to postnatal day 5) for mRNA examination by reverse 
      transcriptase and polymerase chain reaction (RT-PCR) using isozyme specific 
      oligonucleotide primers to the HK1 and HK2 cDNAs. The HK1 mRNA was expressed in 
      both the nonlactating and lactating mammary gland tissue, but HK2 mRNA was found 
      only during lactation. We speculate that the pattern of HK expression might 
      affect human milk production and quality.
CI  - Copyright 1999 Academic Press.
FAU - Kaselonis, G L
AU  - Kaselonis GL
AD  - Department of Pediatrics, UCLA School of Medicine, Los Angeles, California, 
      90095, USA.
FAU - McCabe, E R
AU  - McCabe ER
FAU - Gray, S M
AU  - Gray SM
LA  - eng
GR  - P30 HD27823/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Genet Metab
JT  - Molecular genetics and metabolism
JID - 9805456
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gestational Age
MH  - Hexokinase/*genetics
MH  - Humans
MH  - Isoenzymes/genetics
MH  - Lactation/*metabolism
MH  - Mammary Glands, Animal/*enzymology
MH  - Milk, Human/physiology
MH  - Pregnancy
MH  - Pregnancy, Animal/*metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Transcription, Genetic
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S1096-7192(99)92923-2 [pii]
AID - 10.1006/mgme.1999.2923 [doi]
PST - ppublish
SO  - Mol Genet Metab. 1999 Nov;68(3):371-4. doi: 10.1006/mgme.1999.2923.