PMID- 10564362
OWN - NLM
STAT- MEDLINE
DCOM- 19991208
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 11
IP  - 10
DP  - 1999 Oct
TI  - Axotomy results in major changes in BDNF expression by dorsal root ganglion 
      cells: BDNF expression in large trkB and trkC cells, in pericellular baskets, and 
      in projections to deep dorsal horn and dorsal column nuclei.
PG  - 3539-51
AB  - Brain derived neurotrophic factor (BDNF) is normally expressed by a small number 
      of predominantly trkA-expressing dorsal root ganglion cells. Using 
      immunocytochemistry and in situ hybridization, we have examined the effect of 
      sciatic nerve section on the expression of BDNF in the adult rat. Following 
      axotomy there was a long lasting (4-week) increase in BDNF mRNA and protein in 
      large-diameter, trkB- and trkC-expressing dorsal root ganglion cells. By 2 days 
      postaxotomy, expression of BDNF mRNA had increased from 2% of trkB cells to 50%, 
      and from 18% of trkC cells to 56%. In contrast, BDNF expression in most trkA 
      cells was unchanged, although was increased in the small population of medium- 
      and large-sized trkA cells. Following axotomy, BDNF-immunoreactive terminals 
      appeared in the central axonal projections of large-diameter cells, including the 
      deep dorsal horn and gracile nucleus. Neuropeptide Y was also upregulated 
      following axotomy and was coexpressed with BDNF in the cell bodies and central 
      terminals of the large cells. Ultrastructural analysis in lamina IV of the spinal 
      cord revealed that BDNF terminals in these central projections establish synaptic 
      contacts. Immunoreactivity at 4 weeks was also observed in pericellular baskets 
      that contained calcitonin gene-related peptide (CGRP) and surrounded trkA- and 
      trkB-expressing cells in L4 and L5 lumbar ganglia. These baskets are likely to 
      arise from local, highly immunoreactive, BDNF/CGRP/trkA-expressing cells. Our 
      results identify several novel targets for BDNF and imply that it acts locally in 
      both autocrine and paracrine modes, as well as centrally in a synaptic mode, to 
      modulate the response of somatosensory pathways in nerve injury.
FAU - Michael, G J
AU  - Michael GJ
AD  - Neuroscience Section, Division of Biomedical Sciences, St Bartholomew's and The 
      Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, 
      London, UK.
FAU - Averill, S
AU  - Averill S
FAU - Shortland, P J
AU  - Shortland PJ
FAU - Yan, Q
AU  - Yan Q
FAU - Priestley, J V
AU  - Priestley JV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuropeptide Y)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Afferent Pathways
MH  - Animals
MH  - Autocrine Communication/physiology
MH  - Axotomy
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics
MH  - Cell Size/physiology
MH  - Ganglia, Spinal/chemistry/*cytology
MH  - Gene Expression/physiology
MH  - In Situ Hybridization
MH  - Male
MH  - Microscopy, Electron
MH  - Neuropeptide Y/analysis/genetics
MH  - Pain Threshold/physiology
MH  - Posterior Horn Cells/chemistry/*physiology/ultrastructure
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/*analysis
MH  - Receptor, trkC/*analysis
MH  - Sciatic Nerve/chemistry/cytology/physiology
EDAT- 1999/11/17 00:00
MHDA- 1999/11/17 00:01
CRDT- 1999/11/17 00:00
PHST- 1999/11/17 00:00 [pubmed]
PHST- 1999/11/17 00:01 [medline]
PHST- 1999/11/17 00:00 [entrez]
AID - ejn767 [pii]
AID - 10.1046/j.1460-9568.1999.00767.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 1999 Oct;11(10):3539-51. doi: 10.1046/j.1460-9568.1999.00767.x.