PMID- 10564586
OWN - NLM
STAT- MEDLINE
DCOM- 20000207
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 27
IP  - 1
DP  - 2000 Jan
TI  - A 3-cM commonly deleted region in 6q21 in leukemias and lymphomas delineated by 
      fluorescence in situ hybridization.
PG  - 52-8
AB  - Deletions of the long arm of chromosome 6 (6q) are frequent chromosome 
      aberrations in non-Hodgkin lymphomas (NHLs) and acute lymphoblastic leukemias 
      (ALLs). It is presumed that one or more tumor suppressor genes are localized on 
      6q. By means of fluorescence in situ hybridization (FISH), we attempted to detect 
      and delineate deletions of 6q in leukemias and lymphomas. We performed FISH on 
      148 cases of lymphoma and acute leukemia using a panel of 36 YAC probes 
      distributed from 6q12 to 6q27 and a centromeric probe of chromosome 6 as internal 
      control. Deletions of 6q that included a 7-cM commonly deleted region in 6q21 
      were detected in 59 patients who had B- and T-cell low-grade and high-grade NHL 
      and ALL. FISH with two YAC probes flanking this region was performed on an 
      additional 97 cases of NHL and leukemia. Deletions in 6q21 were detected in an 
      additional 21 cases. In five cases of high-grade B- and T-cell NHL and ALL, the 
      deletion breakpoints were located within the commonly deleted region. To define 
      the deletion breakpoints exactly and to narrow this region further, FISH was 
      performed with six additional YAC probes that have been physically localized 
      within this region. A 3-cM (4-5 Mb) commonly deleted region in 6q21 was 
      delineated. Our study suggests that this commonly deleted region harbors a 
      putative tumor suppressor gene involved in the pathogenesis of both low-grade and 
      high-grade NHL and ALL. Genes Chromosomes Cancer 27:52-58, 2000.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Zhang, Y
AU  - Zhang Y
AD  - Department of Human Genetics, University of Kiel, Kiel, Germany.
FAU - Matthiesen, P
AU  - Matthiesen P
FAU - Harder, S
AU  - Harder S
FAU - Siebert, R
AU  - Siebert R
FAU - Castoldi, G
AU  - Castoldi G
FAU - Calasanz, M J
AU  - Calasanz MJ
FAU - Wong, K F
AU  - Wong KF
FAU - Rosenwald, A
AU  - Rosenwald A
FAU - Ott, G
AU  - Ott G
FAU - Atkin, N B
AU  - Atkin NB
FAU - Schlegelberger, B
AU  - Schlegelberger B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Chromosome Deletion
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - Chromosomes, Human, Pair 6/*genetics
MH  - DNA Probes/genetics
MH  - DNA, Neoplasm/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia/*genetics
MH  - Lymphoma/*genetics
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(200001)27:1<52::AID-GCC7>3.0.CO;2-X [pii]
AID - 10.1002/(sici)1098-2264(200001)27:1<52::aid-gcc7>3.0.co;2-x [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Jan;27(1):52-8. doi: 
      10.1002/(sici)1098-2264(200001)27:1<52::aid-gcc7>3.0.co;2-x.