PMID- 10564591
OWN - NLM
STAT- MEDLINE
DCOM- 20000207
LR  - 20230202
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 27
IP  - 1
DP  - 2000 Jan
TI  - Coamplification of prostate stem cell antigen (PSCA) and MYC in locally advanced 
      prostate cancer.
PG  - 95-103
AB  - Gain of sequences on chromosome arm 8q is a common feature of prostate cancer 
      that may correlate with metastatic and androgen-independent progression. The 
      target gene(s) for this gain is not known, although MYC is amplified in a subset 
      of advanced tumors and is one potential candidate. Prostate stem cell antigen 
      (PSCA) is a prostate-specific cell surface protein that maps to chromosome region 
      8q24.2 and is overexpressed in prostate cancer. Our aim in this study was to test 
      the hypothesis that PSCA overexpression may result from overrepresentation of 
      chromosome arm 8q. Twenty locally advanced prostate cancers were analyzed by 
      dual-probe fluorescence in situ hybridization (FISH) for alterations of MYC and 
      PSCA. Extra copies of MYC were found in 12/20 (60%) tumors, including 5 (25%) 
      with simple gain (no increase in MYC copy number relative to the chromosome 8 
      centromere) and 7 (35%) with an additional increase (AI or overrepresentation) in 
      MYC copy number relative to the centromere. In the five cases with simple gain of 
      MYC, there was a concomitant gain of PSCA. PSCA was overrepresented in 5/7 (71%) 
      cases with AI of MYC. Immunohistochemical staining of the 20 tumors with 
      monoclonal antibodies specific for PSCA showed a high degree of correlation 
      between PSCA gene overrepresentation and protein overexpression. Four of 5 tumors 
      with AI of PSCA overexpressed PSCA protein, compared with only 2/15 tumors with a 
      normal PSCA copy number or simple gain of PSCA (P = 0.014). These results 
      demonstrate that PSCA is co-overrepresented with MYC in a majority of cases, but 
      may not be a necessary part of the 8q amplicon. PSCA protein overexpression can 
      result from AI of PSCA and might be useful as a cell surface marker on prostate 
      cancer cells with 8q overrepresentation. Genes Chromosomes Cancer 27:95-103, 
      2000.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Reiter, R E
AU  - Reiter RE
AD  - Department of Urology, University of California, Los Angeles, CA 90095, USA. 
      rreiter@urology.medsch.ucla.edu
FAU - Sato, I
AU  - Sato I
FAU - Thomas, G
AU  - Thomas G
FAU - Qian, J
AU  - Qian J
FAU - Gu, Z
AU  - Gu Z
FAU - Watabe, T
AU  - Watabe T
FAU - Loda, M
AU  - Loda M
FAU - Jenkins, R B
AU  - Jenkins RB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (PSCA protein, human)
SB  - IM
MH  - Antigens, Neoplasm/genetics
MH  - Biomarkers, Tumor/genetics
MH  - Centromere/genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 8/genetics
MH  - DNA Probes/genetics
MH  - DNA, Neoplasm/genetics
MH  - GPI-Linked Proteins
MH  - Gene Amplification/*genetics
MH  - Gene Dosage
MH  - Genes, myc/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Membrane Glycoproteins/biosynthesis/*genetics
MH  - Neoplasm Proteins/biosynthesis/*genetics
MH  - Prostatic Neoplasms/*genetics
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(200001)27:1<95::AID-GCC12>3.0.CO;2-3 [pii]
AID - 10.1002/(sici)1098-2264(200001)27:1<95::aid-gcc12>3.0.co;2-3 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Jan;27(1):95-103. doi: 
      10.1002/(sici)1098-2264(200001)27:1<95::aid-gcc12>3.0.co;2-3.