PMID- 10565297
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 115
IP  - 1
DP  - 1999 Nov
TI  - Interphase fluorescence in situ hybridization overcomes pitfalls of G-banding 
      analysis with special reference to underestimation of chromosomal aberration 
      rates.
PG  - 32-8
AB  - Fluorescence in situ hybridization (FISH) is suitable for detecting different 
      types of chromosome aberrations on interphase nuclei even in specimens with no or 
      few chromosome metaphases. However, it is not known why FISH is superior to 
      conventional G-banding analysis. The sensitivity of interphase FISH was compared 
      to that of G-banding analysis in 288 leukemia/lymphoma patients for 10 different 
      types of chromosome aberrations: t(9;22) (M- and m-BCR), t(8;21), 11q23 
      abnormalities, t(15;17), del(5)/-5, del(13)/-13, +8, -7, and +12. The results 
      revealed that t(15;17) positive cells could not proliferate well in culture, 
      leading to underestimation of abnormality by G-banding. Monosomy 7 in acute 
      myelocytic leukemia (AML) and myelodysplastic syndrome (MDS) as well as trisomy 
      12 and deletion chromosome 13 in chronic lymphocytic leukemias (CLL) were also 
      severely underestimated by G-banding. On the other hand, no discrepancies were 
      observed in t(8;21), t(9;22), translations involving 11q23, or in trisomy 8. 
      These findings indicate the superiority of interphase FISH over conventional 
      cytogenetics for detecting chromosome abnormalities in small clones, especially 
      for monosomy 7 or (15;17) translocations.
FAU - Tanaka, K
AU  - Tanaka K
AD  - Department of Cancer Cytogenetics, Hiroshima University, Japan.
FAU - Arif, M
AU  - Arif M
FAU - Eguchi, M
AU  - Eguchi M
FAU - Shintani, T
AU  - Shintani T
FAU - Kumaravel, T S
AU  - Kumaravel TS
FAU - Asaoku, H
AU  - Asaoku H
FAU - Kyo, T
AU  - Kyo T
FAU - Dohy, H
AU  - Dohy H
FAU - Kamada, N
AU  - Kamada N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Banding/*methods
MH  - Gene Deletion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Interphase
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/genetics
MH  - Leukemia, Myeloid, Acute/genetics
MH  - Metaphase
MH  - Monosomy
MH  - Myelodysplastic Syndromes/genetics
MH  - Translocation, Genetic
MH  - Trisomy
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S0165460899000795 [pii]
AID - 10.1016/s0165-4608(99)00079-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Nov;115(1):32-8. doi: 10.1016/s0165-4608(99)00079-5.