PMID- 10567563 OWN - NLM STAT- MEDLINE DCOM- 20000106 LR - 20210526 IS - 0270-7306 (Print) IS - 1098-5549 (Electronic) IS - 0270-7306 (Linking) VI - 19 IP - 12 DP - 1999 Dec TI - The AF1 and AF2 domains of the androgen receptor interact with distinct regions of SRC1. PG - 8383-92 AB - The androgen receptor is unusual among nuclear receptors in that most, if not all, of its activity is mediated via the constitutive activation function in the N terminus. Here we demonstrate that p160 coactivators such as SRC1 (steroid receptor coactivator 1) interact directly with the N terminus in a ligand-independent manner via a conserved glutamine-rich region between residues 1053 and 1123. Although SRC1 is capable of interacting with the ligand-binding domain by means of LXXLL motifs, this interaction is not essential since an SRC1 mutant with no functional LXXLL motifs retains its ability to potentiate androgen receptor activity. In contrast, mutants lacking the glutamine-rich region are inactive, indicating that this region is both necessary and sufficient for recruitment of SRC1 to the androgen receptor. This recruitment is in direct contrast to the recruitment of SRC1 to the estrogen receptor, which requires interaction with the ligand-binding domain. FAU - Bevan, C L AU - Bevan CL AD - Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom. FAU - Hoare, S AU - Hoare S FAU - Claessens, F AU - Claessens F FAU - Heery, D M AU - Heery DM FAU - Parker, M G AU - Parker MG LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Mol Cell Biol JT - Molecular and cellular biology JID - 8109087 RN - 0 (Adaptor Proteins, Signal Transducing) RN - 0 (Ligands) RN - 0 (NCOA2 protein, human) RN - 0 (NCOA4 protein, human) RN - 0 (Nuclear Proteins) RN - 0 (Nuclear Receptor Coactivator 2) RN - 0 (Nuclear Receptor Coactivators) RN - 0 (Nuclear Receptor Interacting Protein 1) RN - 0 (Oncogene Proteins) RN - 0 (Receptors, Androgen) RN - 0 (Trans-Activators) RN - 0 (Transcription Factors) RN - 0RH81L854J (Glutamine) RN - EC 2.3.1.48 (Histone Acetyltransferases) RN - EC 2.3.1.48 (NCOA1 protein, human) RN - EC 2.3.1.48 (Nuclear Receptor Coactivator 1) RN - GMW67QNF9C (Leucine) SB - IM MH - Adaptor Proteins, Signal Transducing MH - Amino Acid Motifs MH - Amino Acid Sequence MH - Animals MH - Binding Sites MH - COS Cells MH - Glutamine/metabolism MH - HeLa Cells MH - Histone Acetyltransferases MH - Humans MH - Leucine/metabolism MH - Ligands MH - Molecular Sequence Data MH - Nuclear Proteins/metabolism MH - Nuclear Receptor Coactivator 1 MH - Nuclear Receptor Coactivator 2 MH - Nuclear Receptor Coactivators MH - Nuclear Receptor Interacting Protein 1 MH - *Oncogene Proteins MH - Protein Structure, Tertiary MH - Receptors, Androgen/genetics/*metabolism MH - Trans-Activators/genetics/*metabolism MH - Transcription Factors/genetics/*metabolism PMC - PMC84931 EDAT- 1999/11/24 00:00 MHDA- 1999/11/24 00:01 PMCR- 1999/12/01 CRDT- 1999/11/24 00:00 PHST- 1999/11/24 00:00 [pubmed] PHST- 1999/11/24 00:01 [medline] PHST- 1999/11/24 00:00 [entrez] PHST- 1999/12/01 00:00 [pmc-release] AID - 0764 [pii] AID - 10.1128/MCB.19.12.8383 [doi] PST - ppublish SO - Mol Cell Biol. 1999 Dec;19(12):8383-92. doi: 10.1128/MCB.19.12.8383.