PMID- 10567711
OWN - NLM
STAT- MEDLINE
DCOM- 19991228
LR  - 20211203
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 461
IP  - 3
DP  - 1999 Nov 19
TI  - Potential role of 3-phosphoinositide-dependent protein kinase 1 (PDK1) in 
      insulin-stimulated glucose transporter 4 translocation in adipocytes.
PG  - 277-9
AB  - Insulin stimulation of Glut 4 translocation requires the activation of 
      phosphatidylinositol 3-kinase (PI 3-kinase) but the downstream pathway remains 
      ill-defined. We demonstrated that the overexpression of PDK1 
      (3-phosphoinositide-dependent protein kinase 1), a downstream effector of PI 
      3-kinase, stimulated Glut 4 translocation in adipocytes. This effect does not 
      require the PH domain of PDK1, but expression of the pleckstrin homology 
      domain-deleted PDK1 inhibits the effect of insulin, but not okadaic acid, on Glut 
      4 translocation. These results support a role of the PDK1 pathway in the 
      transmission of insulin signal to Glut translocation.
FAU - Grillo, S
AU  - Grillo S
AD  - Institut National de la Sante et de la Recherche Medicale, INSERM E9911 and 
      INSERM U 145, Faculte de Medecine, Avenue de Valombrose, 06107, Nice, France.
FAU - Gremeaux, T
AU  - Gremeaux T
FAU - Le Marchand-Brustel, Y
AU  - Le Marchand-Brustel Y
FAU - Tanti, J
AU  - Tanti J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Slc2a4 protein, rat)
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - EC 2.7.11.1 (3-Phosphoinositide-Dependent Protein Kinases)
RN  - EC 2.7.11.1 (Pdpk1 protein, rat)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - 3-Phosphoinositide-Dependent Protein Kinases
MH  - Adipocytes/*drug effects/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Biological Transport
MH  - Cell Compartmentation
MH  - Enzyme Induction
MH  - Epitopes/immunology
MH  - Glucose Transporter Type 4
MH  - Insulin/*pharmacology
MH  - Male
MH  - Molecular Sequence Data
MH  - Monosaccharide Transport Proteins/*metabolism
MH  - *Muscle Proteins
MH  - Okadaic Acid/pharmacology
MH  - Protein Serine-Threonine Kinases/chemistry/genetics/*physiology
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Rats, Wistar
MH  - Sequence Deletion
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S0014579399014726 [pii]
AID - 10.1016/s0014-5793(99)01472-6 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Nov 19;461(3):277-9. doi: 10.1016/s0014-5793(99)01472-6.